Rabbit polyclonal BNIP3 antibody (A5683)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal BNIP3 antibody (A5683)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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NIP3 antibody
BCL2 Adenovirus E1B 19kDa Interacting Protein 3 antibody|BCL2/adenovirus E1B 19 kDa protein interacting protein 3 antibody|BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 antibody|BNIP 3 antibody|BNIP3 antibody|BNIP3_HUMAN antibody|NIP 3 antibody|NIP3 antibody|Anti-BNIP3 antibody [ANa40] (ab10433)

SCBT cat No: sc-56167|sc-1715|

Name

Rabbit polyclonal BNIP3 antibody

Catalogue No.

A5683

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human BNIP3

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 21kDa/Observed: Refer to Figures

Formulation

BNIP3 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

BNIP3 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

NIP3 antibody

Database links

Human UniProt/Swiss-Prot:Q12983

Protein names

NIP3

Protein function

Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway.

Protein sequence and domain

Belongs to the NIP3 family.

Protein cellular localization

Mitochondrion. Mitochondrion outer membrane; Single-pass membrane protein. Note: Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. Colocalizes with SPATA18 at the mitochondrion outer membrane.

Background

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. This protein contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein encoded by this gene is known to induce apoptosis, even in the presence of BCL2.

Research area

All research areas>Tumor Suppressor/Apoptosis>BNIP
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize BNIP3 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

Reviews


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Antibody customer reviews & validation data - 145

Validation data ID: 145

Submission date: 2017-03-08

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Result figure:
  • Result figure
  • Result figure

Figure description:

We see a darker band around 21kD in lane 2 (CoCl2-treated C2C12) than in lane 1 (untreated C2C12) as expected, but the band is weaker and the background is higher than a competitor's antibody. No convincing bands seen at the expected size in the other lanes.

Product name

Rabbit polyclonal BNIP3 antibody

Product catalog No.

A5683

Product type

Primary antibody

Supplier

ABclonal

Application type

Western Blot

Antibody specificity rating

Fair

Antibody overall rating

Fair

Your comments

This product showed weaker bands and higher background than a competitor's antibody when run side-by-side with the same samples.
We expect Bnip3 expression in the CoCl2-treated C2C12 sample

Detailed protocol

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