Rabbit polyclonal BRAF antibody (A0038)

$69.00$259.00

Reactivity: Human
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal BRAF antibody (A0038)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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B-RAF1 antibody,BRAF1 antibody,NS7 antibody,RAFB1 antibody,Proto-oncogeneB-Raf antibody,p94 antibody,v-Raf murine sarcoma viral oncogene homolog B1 antibody
FLJ95109 antibody|94 kDa B raf protein antibody|B raf 1 antibody|B Raf proto oncogene serine threonine protein kinase antibody|B Raf proto oncogene, serine/threonine kinase antibody|B RAF1 antibody|B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody|BRAF 1 antibody|BRAF antibody|BRAF_HUMAN antibody|BRAF1 antibody|cRmil antibody|MGC126806 antibody|MGC138284 antibody|Murine sarcoma viral (v-raf) oncogene homolog B1 antibody|Murine sarcoma viral v raf oncogene homolog B1 antibody|NS7 antibody|Oncogen BRAF antibody|oncogene BRAF1 antibody|p94 antibody|Proto-oncogene B-Raf antibody|Proto-oncogene c-Rmil antibody|RAFB 1 antibody|RAFB1 antibody|RMIL antibody|Serine/threonine-protein kinase B-raf antibody|v raf murine sarcoma viral oncogene homolog B antibody|v raf murine sarcoma viral oncogene homolog B1 antibody|v-Raf murine sarcoma viral oncogene homolog B1 antibody|Anti-B Raf antibody [EP152Y] (ab33899)

SCBT cat No: sc-28006|sc-81705|sc-46667|sc-25419|sc-8251|sc-376451|

Name

Rabbit polyclonal BRAF antibody

Catalogue No.

A0038

Reactivity

Human

Immunogen

Recombinant protein of human BRAF

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 94kDa/Observed: Refer to Figures

Formulation

BRAF antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

BRAF antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

B-RAF1 antibody,BRAF1 antibody,NS7 antibody,RAFB1 antibody,Proto-oncogeneB-Raf antibody,p94 antibody,v-Raf murine sarcoma viral oncogene homolog B1 antibody

Database links

Human UniProt/Swiss-Prot:P15056

Protein names

B-RAF1,BRAF1,NS7,RAFB1,Proto-oncogeneB-Raf,p94,v-Raf murine sarcoma viral oncogene homolog B1

Protein function

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Protein tissue specificity

Brain and testis.

Protein sequence and domain

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.; Contains 1 phorbol-ester/DAG-type zinc finger.; Contains 1 protein kinase domain.; Contains 1 RBD (Ras-binding) domain.

Protein post-translational modifications

Phosphorylation at Ser-365 by SGK1 inhibits its activity.; Methylation at Arg-671 decreases stability and kinase activity.; Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.

Protein cellular localization

Nucleus . Cytoplasm. Cell membrane . Note: Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.

Background

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system. It has been observed that MAPK is activated by B-Raf in response to nerve growth factor. More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity. Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Research area

All research areas>Kinases and Phosphatases>Raf
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize BRAF antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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