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Rabbit polyclonal BUB1 antibody (A1929)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC
WB 1:500 – 1:2000 IHC 1:50 – 1:200
Recommended protocols: check protocols
Click or hover above images to see image description for Rabbit polyclonal BUB1 antibody.
Check alternative names for the antibodyExpand
BUB1A antibody, BUB1L antibody, hBUB1 antibody
Bub1 antibody|BUB1 budding uninhibited by benzimidazoles 1 homolog antibody|BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) antibody|BUB1 mitotic checkpoint serine/threonine kinase antibody|BUB1, S. cerevisiae, homolog of antibody|BUB1_HUMAN antibody|BUB1A antibody|BUB1L antibody|Budding uninhibited by benzimidazoles 1 (yeast homolog) antibody|Budding uninhibited by benzimidazoles 1 homolog antibody|Budding uninhibited by benzimidazoles 1, S. cerevisiae, homolog of antibody|hBUB1 antibody|Homolog of mitotic checkpoint gene BUB1 antibody|Mitotic checkpoint gene BUB1 antibody|Mitotic checkpoint serine/threonine protein kinase BUB1 antibody|Mitotic checkpoint serine/threonine-protein kinase BUB1 antibody|Mitotic spindle checkpoint kinase antibody|Putative serine/threonine protein kinase antibody|Anti-Bub1 antibody (ab54893)
SCBT cat No: sc-47743|sc-365685|sc-28257|sc-18286|
Rabbit polyclonal BUB1 antibody
|Catalogue No.|| |
Recombinant protein of human BUB1
|Recommended dilution|| |
WB 1:500 – 1:2000
|Molecular weight|| |
Predicted: 122kDa/Observed: Refer to Figures
BUB1 antibody was tube-contained.
BUB1 antibody was purified using affinity purification.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
BUB1A antibody, BUB1L antibody, hBUB1 antibody
|Database links|| |
|Protein names|| |
BUB1A, BUB1L, hBUB1
|Protein function|| |
Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.
|Protein tissue specificity|| |
High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index.
|Protein sequence and domain|| |
The KEN box is required for its ubiquitination and degradation.; BUB1 N-terminal domain directs kinetochore localization and binding to BUB3.Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.; Contains 1 BUB1 N-terminal domain.; Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Upon spindle-assembly checkpoint activation it is hyperphosphorylated and its kinase activity toward CDC20 is stimulated. Phosphorylation at Thr-609 is required for interaction with PLK1, phosphorylation at this site probably creates a binding site for the POLO-box domain of PLK1, thus enhancing the PLK1-BUB1 interaction.; Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1.
|Protein cellular localization|| |
Nucleus. Chromosome, centromere, kinetochore. Note: Nuclear in interphase cells. Accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. Localizes to the outer kinetochore. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage and occurs very early in prophase. AURKB, CASC5 and INCENP are required for kinetochore localization (By similarity).
The Mitotic Checkpoint Complex (MCC), which contains Bub1, Bub1b, Bub3, Mad2, and Cdc20, controls chromosome segregation and monitors kinetochore-microtubule interactions (1). During mitosis, the MCC complex inhibits the ubiquitin ligase activity of the Anaphase Promoting Complex/Cyclosome (APC/C), thereby preventing cells with unaligned chromosomes from prematurely entering anaphase (2). Research studies have shown that Bub1b and Bub1 kinases are mutated in several types of human malignancies including hematopoietic, colorectal, lung, and breast cancers (3). Biallelic mutations in Bub1b have been found in mosaic variegated aneuploidy syndrome and premature chromatid separation syndrome (4). Bub1b mouse germline knockouts are embryonic lethal with heterozygous animals displaying genetic instability, early aging phenotypes, and increased cancer susceptibility (5). Bub3 binds both Bub1 and Bub1b, facilitating their recruitment to kinetochores (6), and is required for functional microtubule-kinetochore interactions (7).
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|Product type|| |
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