Rabbit polyclonal BUB3 antibody (A6536)


Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal BUB3 antibody (A6536)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal BUB3 antibody.

Alternative names:

Check alternative names for the antibody


BUB3L antibody, hBUB3 antibody
Aa2-050 antibody|AU019800 antibody|AU021329 antibody|AU043350 antibody|AW146323 antibody|BUB 3 antibody|BUB 3L antibody|BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog antibody|bub3 antibody|BUB3 budding uninhibited by benzimidazoles 3 homolog antibody|BUB3 mitotic checkpoint protein antibody|BUB3_HUMAN antibody|BUB3L antibody|Budding uninhibited by benomyl antibody|Budding uninhibited by benzimidazoles 3 antibody|Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae) antibody|Budding uninhibited by benzimidazoles 3 homolog (yeast) antibody|Budding uninhibited by benzimidazoles 3 homolog antibody|Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of antibody|C78067 antibody|hBUB3 antibody|Mitotic checkpoint component antibody|Mitotic checkpoint protein bub3 antibody|OTTHUMP00000020679 antibody|OTTHUMP00000020680 antibody|WD repeat type I transmembrane protein A72.5 antibody|Anti-Bub3 antibody [EPR5319(2)] (ab133699)

SCBT cat No: sc-136217|sc-19397|sc-376506|sc-28258|


Rabbit polyclonal BUB3 antibody

Catalogue No.



Human, Mouse, Rat


Recombinant protein of human BUB3





Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200







Molecular weight

Predicted: 37kDa/Observed: Refer to figures


BUB3 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.


BUB3 antibody was purified using affinity purification.


Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

BUB3L antibody, hBUB3 antibody

Database links

Human UniProt/Swiss-Prot:O43684

Protein names


Protein function

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.

Protein sequence and domain

Belongs to the WD repeat BUB3 family.; Contains 7 WD repeats.

Protein post-translational modifications

Poly-ADP-ribosylated by PARP1.

Protein cellular localization

Nucleus. Chromosome, centromere, kinetochore . Note: Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.


This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Research area

All research areas>Cell Cycle Proteins>BUB
(View all antibody categories related to Cell Cycle Proteins)


AntibodyPlus can customize BUB3 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody


ABclonal Inc.


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