Rabbit polyclonal CAD antibody (A8344)

$69.00$259.00

Reactivity: Human
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal CAD antibody (A8344)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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Aspartate carbamoyltransferase antibody|Aspartate transcarbamylase antibody|CAD protein antibody|CAD trifunctional protein antibody|Carbamoyl phosphate synthetase 2 antibody|CPSase ATCase DHOase antibody|Dihydroorotase antibody|Glutamine dependent carbamoyl phosphate synthase antibody|Multifunctional protein CAD antibody|Anti-CAD antibody [EP710Y] (ab40800)

SCBT cat No: sc-10522|sc-376072|sc-30061|sc-10521|sc-166282|sc-17290|

Name

Rabbit polyclonal CAD antibody

Catalogue No.

A8344

Reactivity

Human

Immunogen

Recombinant protein of human CAD

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 243kDa/Observed: Refer to figures

Formulation

CAD antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

CAD antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Database links

Human UniProt/Swiss-Prot:P27708

Protein function

This protein is a “fusion” protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).

Protein sequence and domain

In the central section; belongs to the DHOase family.; Contains 2 ATP-grasp domains.; Contains 1 glutamine amidotransferase type-1 domain.

Protein post-translational modifications

Activated by MAP kinase (Erk1/2) phosphorylation just prior to the S phase of the cell cycle, when the demand for pyrimidine nucleotides is greatest, and down-regulated as the cells emerge from S phase by protein kinase A (PKA) phosphorylation. Phosphorylation at Ser-1859 by RPS6KB1 downstream of MTOR promotes oligomerization and stimulates dihydroorotase activity. Phosphorylation at Ser-1406 reduces sensitivy to feedback inhibition by UTP.

Protein cellular localization

Cytoplasm . Nucleus . Note: Cytosolic and unphosphorylated in resting cells, translocates to the nucleus in response to EGF stimulation, nuclear import promotes optimal cell growth.

Background

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms.

Note

AntibodyPlus can customize CAD antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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