Rabbit polyclonal CALD1 antibody (A5366)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal CALD1 antibody (A5366)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal CALD1 antibody.

Alternative names:

Check alternative names for the antibody

Expand

CDM antibody, HCAD antibody, LCAD antibody, H-CAD antibody, L-CAD antibody, NAG22 antibody
CAD antibody|CALD 1 antibody|CALD1 antibody|CALD1_HUMAN antibody|Caldesmon 1 antibody|Caldesmon 1 Isoform 1 antibody|Caldesmon 1 Isoform 2 antibody|Caldesmon 1 Isoform 3 antibody|Caldesmon 1 Isoform 4 antibody|Caldesmon 1 Isoform 5 antibody|Caldesmon antibody|Caldesmon1 antibody|CDM antibody|H CAD antibody|HCAD antibody|L CAD antibody|LCAD antibody|MGC21352 antibody|NAG22 antibody|Anti-Caldesmon antibody [E89] (ab32330)

SCBT cat No: sc-135965|sc-271222|sc-7574|sc-58700|sc-15374|sc-7575|sc-130401|sc-390250|sc-98427|sc-48352|

Name

Rabbit polyclonal CALD1 antibody

Catalogue No.

A5366

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human CALD1

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 63kDa/Observed: Refer to Figures

Formulation

CALD1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

CALD1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CDM antibody, HCAD antibody, LCAD antibody, H-CAD antibody, L-CAD antibody, NAG22 antibody

Database links

Human UniProt/Swiss-Prot:Q05682

Protein names

CDM, HCAD, LCAD, H-CAD, L-CAD, NAG22

Protein function

Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity).

Protein tissue specificity

High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.

Protein sequence and domain

The N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.Belongs to the caldesmon family.

Protein post-translational modifications

In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization. CDK1-mediated phosphorylation promotes Schwann cell migration during peripheral nerve regeneration (By similarity).

Protein cellular localization

Cytoplasm, cytoskeleton . Cytoplasm, myofibril . Note: On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).

Background

This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Research area

All research areas>Structural Proteins>Caldesmon
(View all antibody categories related to Structural Proteins)

Note

AntibodyPlus can customize CALD1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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