Rabbit polyclonal CASP14 antibody (A6541)

$69.00$259.00

Reactivity: Human
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal CASP14 antibody (A6541)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:10 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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CASP-5 antibody|CASP5 antibody|CASP5_HUMAN antibody|Caspase-5 subunit p10 antibody|ICE(rel)-III antibody|ICERELIII antibody|ICH 3 antibody|ICH 3 protease antibody|Protease ICH-3 antibody|Protease TY antibody|TY antibody|TY protease antibody|Anti-Caspase-14 antibody [EPR12927] (ab174847)

SCBT cat No: sc-136351|sc-56040|sc-48395|sc-48336|sc-5628|sc-515259|sc-9644|

Name

Rabbit polyclonal CASP14 antibody

Catalogue No.

A6541

Reactivity

Human

Immunogen

Recombinant protein of human CASP14

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:10 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 27kDa/Observed: Refer to figures

Formulation

CASP14 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

CASP14 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Database links

Human UniProt/Swiss-Prot:P31944

Protein function

Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum . Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin (By similarity). In vitro has a preference for the substate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF . Involved in processing of prosaposin in the epidermis (By similarity). May be involved in retinal pigment epithelium cell barrier function . Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD .

Protein tissue specificity

Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only expressed at very low levels . The p17/10 mature form is expressed in epidermis stratum corneum, the p20/p8 intermediate form in epidermis upper granular cells of the stratum granulosum .

Protein sequence and domain

Belongs to the peptidase C14A family.

Protein post-translational modifications

Maturation by proteolytic processing appears to be a two-step process. The precursor is processed by KLK7 to yield the p20/p8 intermediate form which acts on the precursor to yield the p17/p10 mature form . Initially, cleavage between Ile-152 and Lys-153 has been proposed to yield the large and small subunits of the active enzyme .

Protein cellular localization

Cytoplasm . Nucleus .

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier.

Research area

All research areas>Tumor Suppressor/Apoptosis>Caspase
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize CASP14 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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