Rabbit polyclonal CASP3 antibody (A0214)

Rabbit polyclonal CASP3 antibody

Human, Mouse, Rat

Rabbit

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Unconjugated

Predicted: 32kDa/Observed: Refer to Figures

CASP3 antibody was purified using affinity purification.

CASP3 antibody,CPP32 antibody,CPP32B antibody,SCA-1 antibody,Caspase-3 antibody,APOPAIN antibody

CASP3,CPP32,CPP32B,SCA-1,Caspase-3,APOPAIN

Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.; S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).

AntibodyPlus can customize CASP3 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

ABclonal Inc.