Rabbit polyclonal CASP3 antibody (A3164)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal CASP3 antibody (A3164)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:1000 IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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CPP32 antibody, SCA-1 antibody, CPP32B antibody
A830040C14Rik antibody|Caspase-3 subunit p12 antibody|CC3 antibody|CPP-32 antibody|CPP32 antibody|CPP32B antibody|Cysteine protease CPP32 antibody|EC 3.4.22.56 antibody|LICE antibody|mldy antibody|OTTHUMP00000165052 antibody|OTTHUMP00000165053 antibody|OTTHUMP00000165054 antibody|PARP cleavage protease antibody|Procaspase3 antibody|Protein Yama antibody|SCA 1 antibody|SCA-1 antibody|SREBP cleavage activity 1 antibody|Yama antibody|Anti-Caspase-3 antibody (ab13847)

SCBT cat No: sc-56053|sc-70497|sc-65496|sc-56046|sc-70498|sc-65497|sc-136219|sc-56050|sc-56051|sc-56052|sc-7272|sc-7148|sc-1225|sc-271759|sc-1224|sc-271028|sc-373730|sc-166589|sc-98785|sc-22139|sc-22140|sc-1226|sc-56056|sc-1229|

Name

Rabbit polyclonal CASP3 antibody

Catalogue No.

A3164

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human CASP3

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:1000
IHC 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 32kDa/Observed: Refer to Figures

Formulation

CASP3 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

CASP3 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CPP32 antibody, SCA-1 antibody, CPP32B antibody

Database links

Human UniProt/Swiss-Prot:P42574

Protein names

CPP32, SCA-1, CPP32B

Protein function

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a ‘216-Asp-, -Gly-217’ bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

Protein tissue specificity

Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

Protein sequence and domain

Belongs to the peptidase C14A family.

Protein post-translational modifications

Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.; S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.

Protein cellular localization

Cytoplasm.

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer’s disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Research area

All research areas>Tumor Suppressor/Apoptosis>Caspase
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize CASP3 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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