Rabbit polyclonal CASP8 antibody (A0215)

$69.00$259.00

Reactivity: Human,Mouse
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal CASP8 antibody (A0215)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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ALPS2B antibody, CAP4 antibody, Casp-8 antibody, FLICE antibody, FLJ17672 antibody, MACH antibody, MCH5 antibody, MGC78473 antibody, Caspase8 antibody
ALPS2B antibody|Caspase-8 subunit p10 antibody|CED 3 antibody|FADD Like ICE antibody|FADD-homologous ICE/CED-3-like protease antibody|FADD-like ICE antibody|FLICE antibody|FLJ17672 antibody|ICE-like apoptotic protease 5 antibody|MACH alpha 1/2/3 protein antibody|MACH antibody|MACH beta 1/2/3/4 protein antibody|MCH5 antibody|MGC78473 antibody|MORT1 associated ced 3 homolog antibody|MORT1-associated CED-3 homolog antibody|OTTHUMP00000163717 antibody|OTTHUMP00000163720 antibody|OTTHUMP00000163724 antibody|OTTHUMP00000163725 antibody|OTTHUMP00000165062 antibody|OTTHUMP00000165063 antibody|OTTHUMP00000165064 antibody|OTTHUMP00000206552 antibody|OTTHUMP00000206582 antibody|Anti-Caspase-8 antibody (ab25901)

SCBT cat No: sc-81656|sc-81657|sc-70501|sc-73526|sc-70502|sc-81661|sc-81662|sc-56070|sc-6133|sc-56072|sc-56071|sc-6136|sc-393776|sc-5263|sc-166320|sc-166596|sc-7890|sc-6134|

Name

Rabbit polyclonal CASP8 antibody

Catalogue No.

A0215

Reactivity

Human, Mouse

Immunogen

Recombinant protein of human CASP8

Host

Rabbit

Applications

WB

Recommended dilution

WB 1:500 – 1:2000

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 62kDa/Observed: Refer to Figures

Formulation

CASP8 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

CASP8 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

ALPS2B antibody, CAP4 antibody, Casp-8 antibody, FLICE antibody, FLJ17672 antibody, MACH antibody, MCH5 antibody, MGC78473 antibody, Caspase8 antibody

Database links

Human UniProt/Swiss-Prot:Q14790

Protein names

ALPS2B, CAP4, Casp-8, FLICE, FLJ17672, MACH, MCH5, MGC78473, Caspase8

Protein function

Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-, -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

Protein tissue specificity

Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.

Protein sequence and domain

Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.Belongs to the peptidase C14A family.; Contains 2 DED (death effector) domains.

Protein post-translational modifications

Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.; Phosphorylation on Ser-387 during mitosis by CDK1 inhibits activation by proteolysis and prevents apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes.

Protein cellular localization

Cytoplasm.

Background

Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.

Research area

All research areas>Tumor Suppressor/Apoptosis>Caspase
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize CASP8 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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