Rabbit Polyclonal Cbl antibody (STJ92057)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Cbl antibody (STJ92057)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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CBL antibody, CBL2 antibody, RNF55 antibody,|4732447J05Rik antibody|C CBL antibody|Cas Br M (murine) ecotropic retroviral transforming sequence antibody|Casitas B lineage lymphoma proto oncogene antibody|Casitas B-lineage lymphoma proto-oncogene antibody|CBL 2 antibody|cbl antibody|CBL_HUMAN antibody|CBL2 antibody|E3 ubiquitin protein ligase CBL antibody|E3 ubiquitin-protein ligase CBL antibody|Oncogene CBL2 antibody|Proto oncogene c CBL antibody|Proto-oncogene c-CBL antibody|RGD1561386 antibody|RING finger protein 55 antibody|RNF55 antibody|Signal transduction protein CBL antibody|Phospho anti-CBL (Y774) antibody [E160] (ab32446)
SCBT cat No: sc-1651|sc-170|sc-393543|sc-398282|sc-8372|sc-390648|sc-390649|

 

Name

Cbl Polyclonal Antibody

Catalogue No.

STJ92057

Reactivity

Human, Mouse, Rat

Specificity

Cbl Polyclonal Antibody detects endogenous levels of Cbl protein.

Immunogen

Synthesized peptide derived from Cbl at AA range 610-690

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

120 kDa

Formulation

Cbl Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Cbl Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

E3 ubiquitin-protein ligase CBL antibody, Casitas B-lineage lymphoma proto-oncogene antibody, Proto-oncogene c-Cbl antibody, RING finger protein 55 antibody, Signal transduction protein CBL antibody

Database links

Human UniProt/Swiss-Prot:P22681;Mouse UniPort/Swiss-Prot: P22682;Rat UniProt/Swiss-Port: D3ZV15;Human Entrez Gene: 867;Mouse Entrez Gene: 12402;

Protein names

E3 ubiquitin-protein ligase CBL , Casitas B-lineage lymphoma proto-oncogene , Proto-oncogene c-Cbl , RING finger protein 55 , Signal transduction protein CBL

Protein function

Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The ‘Tyr-731’ phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. /

Involvement in disease

Noonan syndrome-like disorder with or without juvenile myelomonocytic leukemia (NSLL) [MIM:613563]: A syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects. Some have an increased risk for certain malignancies, particularly juvenile myelomonocytic leukemia. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme. / The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain. / Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain. / Contains 1 RING-type zinc finger. / Contains 1 UBA domain.

Protein post-translational modifications

Phosphorylated on tyrosine residues by ALK, EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues in response to FLT1 and KIT signaling. Phosphorylated on tyrosine residues by INSR and FGR. Phosphorylated on several tyrosine residues by constitutively activated FGFR3. Not phosphorylated at Tyr-731 by FGFR3. Phosphorylated on tyrosine residues by activated CSF1R, PDGFRA and PDGFRB. Phosphorylated on tyrosine residues by HCK. / Ubiquitinated, leading to its degradation via the proteasome.

Protein cellular localization

Cytoplasm / Cell membrane

Research area

All research areas>Signaling Intermediates>Cbl
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus can customize Cbl Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


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Antibody customer reviews & validation data - 55

Validation data ID: 55

Submission date: 2017-01-10

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Result figure:
Result figure

Figure description:

The figure showing the expression of CBL in LY3 and DHL2. CBL expression is low in LY3 and overexpressed in DHL2.

Product name

Rabbit Polyclonal Cbl antibody

Product catalog No.

STJ92057

Product type

Primary antibody

Supplier

St John's Laboratory Ltd

Application type

Western Blot

Antibody specificity rating

Good

Antibody overall rating

Excellent

Your comments

Its working good in Lymphoma cell lines.

Detailed protocol

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