Rabbit polyclonal CBX5 antibody (A1098)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF,IP,ChIP
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal CBX5 antibody (A1098)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF,IP,ChIP

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200 IP 1:50 – 1:200 ChIP 1:20 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal CBX5 antibody.

Alternative names:

Check alternative names for the antibody

Expand

CBX5 antibody,HP1 antibody,HP1A antibody
Antigen p25 antibody|CBX5 antibody|CBX5_HUMAN antibody|CG8409 antibody|Chromobox 5 antibody|Chromobox homolog 5 (HP1 alpha homolog, Drosophila) antibody|Chromobox homolog 5 antibody|Chromobox protein homolog 5 antibody|Epididymis luminal protein 25 antibody|HEL25 antibody|Heterochromatin protein 1 alpha antibody|Heterochromatin protein 1 antibody|Heterochromatin protein 1 homolog alpha antibody|HP1 alpha antibody|HP1 alpha homolog antibody|HP1 antibody|HP1A antibody|HP1Hs alpha antibody|Su(var)205 antibody|Anti-HP1 alpha antibody – ChIP Grade (ab77256)

SCBT cat No: sc-10217|sc-10215|sc-28735|sc-10210|sc-30742|sc-130446|sc-30743|

Name

Rabbit polyclonal CBX5 antibody

Catalogue No.

A1098

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human CBX5

Host

Rabbit

Applications

WB, IHC, IF, IP, ChIP

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200
IP 1:50 – 1:200
ChIP 1:20 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 22kDa/Observed: Refer to Figures

Formulation

CBX5 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

CBX5 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CBX5 antibody,HP1 antibody,HP1A antibody

Database links

Human UniProt/Swiss-Prot:P45973

Protein names

CBX5,HP1,HP1A

Protein function

Component of heterochromatin that recognizes and binds histone H3 tails methylated at ‘Lys-9’ (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when ‘Tyr-41’ of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Protein sequence and domain

Contains 2 chromo domains.

Protein post-translational modifications

Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.; Ubiquitinated.

Protein cellular localization

Nucleus . Chromosome . Chromosome, centromere . Note: Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.

Background

Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure (1). All three HP1 family members (alpha, beta, and gamma) are primarily associated with centromeric heterochromatin; however, HP1beta and gamma also localize to euchromatic sites in the genome (2,3). HP1 proteins are approximately 25 kDa in size and contain a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated at Lys9, a histone “mark” closely associated with centromeric heterochromatin (4,5). The variable hinge region binds both RNA and DNA in a sequence-independent manner (6). The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases, and the p150 subunit of chromatin-assembly factor-1 (CAF1) (7-9). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells (10,11). HP1 proteins are subject to multiple types of post-translational modifications, including phosphorylation, acetylation, methylation, ubiquitination, and sumoylation, suggesting multiple means of regulation (12-14).

Research area

All research areas>Transcription Regulators>HP1
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize CBX5 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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