Rabbit polyclonal CCNB1 antibody (A2056)

$69.00$259.00

Reactivity: Human
Applications: WB,IHC,ICC,IF,IP
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal CCNB1 antibody (A2056)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,ICC,IF,IP

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 ICC 1:50 – 1:200 IF 1:50 – 1:200 IP 1:20 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal CCNB1 antibody.

Alternative names:

Check alternative names for the antibody

Expand

CCNB1 antibody, CCNB antibody, cyclin B1 antibody
CCNB 1 antibody|CCNB antibody|ccnb1 antibody|CCNB1_HUMAN antibody|Cyclin B1 antibody|G2 mitotic specific cyclin B1 antibody|G2/mitotic-specific cyclin-B1 antibody|Anti-Cyclin B1 antibody [V152] (ab72)

SCBT cat No: sc-245|

Name

Rabbit polyclonal CCNB1 antibody

Catalogue No.

A2056

Reactivity

Human

Immunogen

A synthetic peptide of human CCNB1

Host

Rabbit

Applications

WB, IHC, ICC, IF, IP

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
ICC 1:50 – 1:200
IF 1:50 – 1:200
IP 1:20 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 48kDa/Observed: Refer to Figures

Formulation

CCNB1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

CCNB1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CCNB1 antibody, CCNB antibody, cyclin B1 antibody

Database links

Human UniProt/Swiss-Prot:P14635

Protein names

CCNB1, CCNB, cyclin B1

Protein function

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

Protein sequence and domain

Belongs to the cyclin family. Cyclin AB subfamily.

Protein post-translational modifications

Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.; Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.

Protein cellular localization

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Background

Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) (1,2) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C (3-5). Four cyclin B1 phosphorylation sites (Ser126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis (6-9). While MPF itself can phosphorylate Ser126 and Ser128, polo-like kinase 1 (PLK1) phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 (6,10). At the end of mitosis, cyclin B1 is targeted for degradation by the anaphase-promoting complex (APC), allowing for cell cycle progression (11). Research studies have shown that cyclin B1 is overexpressed in breast, prostate, and non-small cell lung cancers (12-14).

Research area

All research areas>Cell Cycle Proteins>Cyclin
(View all antibody categories related to Cell Cycle Proteins)

Note

AntibodyPlus can customize CCNB1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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