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Rabbit polyclonal CCNB1 antibody (A2056)
$69.00–$259.00
Reactivity: Human
Applications: WB,IHC,ICC,IF,IP
Conjugation: Unconjugated
Supplier: ABclonal Inc.
Rabbit polyclonal CCNB1 antibody (A2056)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC,ICC,IF,IP
Recommended dilution:
WB 1:500 – 1:2000 IHC 1:50 – 1:200 ICC 1:50 – 1:200 IF 1:50 – 1:200 IP 1:20 – 1:100
Recommended protocols: check protocols
Image descriptions:
Click or hover above images to see image description for Rabbit polyclonal CCNB1 antibody.
Alternative names:
Check alternative names for the antibody
ExpandCCNB1 antibody, CCNB antibody, cyclin B1 antibody
CCNB 1 antibody|CCNB antibody|ccnb1 antibody|CCNB1_HUMAN antibody|Cyclin B1 antibody|G2 mitotic specific cyclin B1 antibody|G2/mitotic-specific cyclin-B1 antibody|Anti-Cyclin B1 antibody [V152] (ab72)
SCBT cat No: sc-245|
Name | Rabbit polyclonal CCNB1 antibody |
---|---|
Catalogue No. | A2056 |
Reactivity | Human |
Immunogen | A synthetic peptide of human CCNB1 |
Host | Rabbit |
Applications | WB, IHC, ICC, IF, IP |
Recommended dilution | WB 1:500 – 1:2000 |
Clonality | Polyclonal |
Conjugation | Unconjugated |
Isotype | IgG |
Molecular weight | Predicted: 48kDa/Observed: Refer to Figures |
Formulation | CCNB1 antibody was tube-contained. |
Purification | CCNB1 antibody was purified using affinity purification. |
Storage | Store at -20 Celsius degree. Avoid freeze / thaw cycles. |
Alternative antibody names | CCNB1 antibody, CCNB antibody, cyclin B1 antibody |
Database links | Human UniProt/Swiss-Prot:P14635 |
Protein names | CCNB1, CCNB, cyclin B1 |
Protein function | Essential for the control of the cell cycle at the G2/M (mitosis) transition. |
Protein sequence and domain | Belongs to the cyclin family. Cyclin AB subfamily. |
Protein post-translational modifications | Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.; Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site. |
Protein cellular localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. |
Background | Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) (1,2) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C (3-5). Four cyclin B1 phosphorylation sites (Ser126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis (6-9). While MPF itself can phosphorylate Ser126 and Ser128, polo-like kinase 1 (PLK1) phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 (6,10). At the end of mitosis, cyclin B1 is targeted for degradation by the anaphase-promoting complex (APC), allowing for cell cycle progression (11). Research studies have shown that cyclin B1 is overexpressed in breast, prostate, and non-small cell lung cancers (12-14). |
Research area | All research areas>Cell Cycle Proteins>Cyclin |
Note | AntibodyPlus can customize CCNB1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation. |
Product type | Primary antibody |
Supplier | ABclonal Inc. |
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