Rabbit Polyclonal CD63 antibody (STJ96890)

$99.00$319.00

Reactivity: Human
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal CD63 antibody (STJ96890)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC-P 1:100-1:300; ELISA 1:20000;

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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CD63 antibody, MLA1 antibody, TSPAN30 antibody,|Lysosomal associated membrane protein 3 antibody|CD 63 antibody|CD63 antibody|CD63 antigen (melanoma 1 antigen) antibody|CD63 antigen antibody|CD63 antigen melanoma 1 antigen antibody|CD63 molecule antibody|CD63_HUMAN antibody|gp55 antibody|Granulophysin antibody|LAMP 3 antibody|LAMP-3 antibody|LAMP3 antibody|LIMP antibody|Lysosomal-associated membrane protein 3 antibody|Lysosome associated membrane glycoprotein 3 antibody|Mast cell antigen AD1 antibody|ME491 antibody|Melanoma 1 antigen antibody|Melanoma associated antigen ME491 antibody|Melanoma associated antigen MLA1 antibody|Melanoma-associated antigen ME491 antibody|MGC72893 antibody|MLA 1 antibody|MLA1 antibody|NGA antibody|Ocular melanoma associated antigen antibody|Ocular melanoma-associated antigen antibody|OMA81H antibody|PTLGP40 antibody|Tetraspanin 30 antibody|Tetraspanin-30 antibody|Tspan 30 antibody|Tspan-30 antibody|TSPAN30 antibody|Anti-CD63 antibody [TS63] (ab59479)
SCBT cat No: sc-71493|sc-66166|sc-59284|sc-365604|sc-31210|sc-15363|sc-7080|sc-5275|sc-59286|sc-31213|sc-25183|

Name

CD63 Polyclonal Antibody

Catalogue No.

STJ96890

Reactivity

Human

Specificity

CD63 Polyclonal Antibody detects endogenous levels of CD63 protein.

Immunogen

Synthesized peptide derived from CD63 antigen at AA range 121-170

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC-P 1:100-1:300; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Formulation

CD63 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

CD63 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

CD63 antigen antibody, Granulophysin antibody, Lysosomal-associated membrane protein 3 antibody, LAMP-3 antibody, Melanoma-associated antigen ME491 antibody, OMA81H antibody, Ocular melanoma-associated antigen antibody, Tetraspanin-30 antibody, Tspan-30 antibody, CD antigen CD63 antibody

Database links

Human UniProt/Swiss-Prot:P08962;Mouse UniPort/Swiss-Prot: P41731;Rat UniProt/Swiss-Port: P28648;Human Entrez Gene: 967;Mouse Entrez Gene: 12512;Rat Entrez Gene: Rn.11068

Protein names

CD63 antigen , Granulophysin , Lysosomal-associated membrane protein 3 , LAMP-3 , Melanoma-associated antigen ME491 , OMA81H , Ocular melanoma-associated antigen , Tetraspanin-30 , Tspan-30 , CD antigen CD63

Protein function

Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.

Protein tissue specificity

Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.

Protein sequence and domain

Belongs to the tetraspanin (TM4SF) family.

Protein post-translational modifications

Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro).

Protein cellular localization

Cell membrane; Multi-pass membrane protein / Lysosome membrane; Multi-pass membrane protein / Late endosome membrane; Multi-pass membrane protein / Endosome > multivesicular body / Melanosome / Secreted > exosome

Research area

All research areas>Lymphocyte Signaling>CD63
(View all antibody categories related to Lymphocyte Signaling)

Note

AntibodyPlus can customize CD63 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human breast cancer tissue

1: CD63 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human breast cancer tissue

1: CD63 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human stomach cancer tissue

1: CD63 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human stomach cancer tissue

1: CD63 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human breast cancer tissue

1: CD63 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver tissue

1: CD63 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1: CD63 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human lung tissue

1: CD63 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human lung cancer tissue

1: CD63 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human kidney tissue

1: CD63 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human kidney cancer tissue

1: CD63 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: CD63 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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