Rabbit polyclonal CD86 antibody (A1199)


Reactivity: Human,Mouse
Applications: WB,IHC,ICC,IF,IP
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal CD86 antibody (A1199)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,ICC,IF,IP

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 ICC 1:50 – 1:100 IF 1:50 – 1:100 IP 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody


CD86 antibody,B7-2 antibody,B70 antibody,CD28LG2 antibody,LAB72 antibody,MGC34413 antibody
Activation B7-2 antigen 3 antibody|Activation B7-2 antigen antibody|B-lymphocyte activation antigen B7-2 2 antibody|B-lymphocyte activation antigen B7-2 antibody|B7 2 antibody|B7 antibody|B7-2 antibody|B7.2 antibody|B70 antibody|B72 antigen antibody|BU63 antibody|CD28 antigen ligand 2 2 antibody|CD28 antigen ligand 2 antibody|Cd28l2 antibody|CD28LG2 antibody|CD86 antibody|CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody|CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody|CD86 antigen antibody|CD86 molecule antibody|CD86_HUMAN antibody|CLS1 antibody|CTLA-4 counter-receptor B7.2 2, 3 antibody|CTLA-4 counter-receptor B7.2 antibody|Early T-cell costimulatory molecule 1 antibody|ETC-1 antibody|FUN 1 antibody|FUN-1 antibody|LAB72 antibody|Ly-58 antibody|Ly58 antibody|MB7 antibody|MB7-2 antibody|Membrane glycoprotein antibody|MGC34413 antibody|T-lymphocyte activation antigen CD86 antibody|TS/A-2 antibody|Anti-CD86 antibody [EP1158Y] (ab53004)

SCBT cat No: sc-58915|sc-19617|sc-1635|sc-28347|sc-52448|sc-9092|


Rabbit polyclonal CD86 antibody

Catalogue No.



Human, Mouse


Recombinant protein of human CD86





Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
ICC 1:50 – 1:100
IF 1:50 – 1:100
IP 1:50 – 1:100







Molecular weight

Predicted: 38kDa/Observed: Refer to Figures


CD86 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.


CD86 antibody was purified using affinity purification.


Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CD86 antibody,B7-2 antibody,B70 antibody,CD28LG2 antibody,LAB72 antibody,MGC34413 antibody

Database links

Human UniProt/Swiss-Prot:P42081

Protein names


Protein function

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.; (Microbial infection) Acts as a receptor for adenovirus subgroup B.

Protein tissue specificity

Expressed by activated B-lymphocytes and monocytes.

Protein sequence and domain

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.; Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Protein post-translational modifications

Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.

Protein cellular localization

Cell membrane; Single-pass type I membrane protein.


This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Research area

All research areas>Lymphocyte Signaling>B7-2
(View all antibody categories related to Lymphocyte Signaling)


AntibodyPlus can customize CD86 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody


ABclonal Inc.


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Antibody customer reviews & validation data - 144

Validation data ID: 144

Submission date: 2017-03-06

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Result figure:
  • Result figure
  • Result figure

Figure description:

CD86 Rabbit polyclonal antibody was incubated for 60 minutes at a 1:400 concentration, in both human mammary gland tissue (adjacent to tumor) and human tonsil tissue.

Product name

CD86 Rabbit Polyclonal Antibody

Product catalog No.


Product type

Primary antibody



Application type

Immunohistochemistry (IHC)

Antibody specificity rating


Antibody overall rating


Your comments

The ABClonal CD86 was testing in human mammary gland tissue and human tonsil, with antigen retrieval conditions of 115 deg C, 20 minutes under pressure in EDTA antigen retrieval buffer (pH 9). The antibody has been tested at a 1:400 dilution, which still seems to be too concentrated for our application; however, we are still testing it and intend to dilute it further. Darker stained cells do appear in our tissue, although the background is still too high. The staining is deep and crisp.

Detailed protocol

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