Rabbit polyclonal CDC34 antibody (A5457)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Rabbit polyclonal CDC34 antibody (A5457)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal CDC34 antibody.

Alternative names:

Check alternative names for the antibody

Expand

UBC3 antibody, UBCH3 antibody, UBE2R1 antibody, E2-CDC34 antibody
Cdc 34 antibody|Cdc34 antibody|Cell division cycle 34 antibody|Cell division cycle 34 homolog (S. cerevisiae) antibody|Cell division cycle 34 homolog antibody|E2 CDC34 antibody|UB2R1_HUMAN antibody|UBC 3 antibody|UBC3 antibody|UBCH3 antibody|UBE2 R1 antibody|UBE2R1 antibody|Ubiquitin carrier protein antibody|Ubiquitin conjugating enzyme Cdc34 antibody|Ubiquitin conjugating enzyme E2 32 kDa complementing antibody|Ubiquitin protein ligase antibody|Ubiquitin protein ligase R1 antibody|Ubiquitin-conjugating enzyme E2 R1 antibody|Ubiquitin-conjugating enzyme E2-32 kDa complementing antibody|Ubiquitin-conjugating enzyme E2-CDC34 antibody|Ubiquitin-protein ligase R1 antibody|Anti-Cdc34 antibody [EPR16808] (ab204515)

SCBT cat No: sc-135861|sc-166738|sc-28381|sc-5616|

Name

Rabbit polyclonal CDC34 antibody

Catalogue No.

A5457

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human CDC34

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 34kDa/Observed: Refer to Figures

Formulation

CDC34 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

CDC34 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

UBC3 antibody, UBCH3 antibody, UBE2R1 antibody, E2-CDC34 antibody

Database links

Human UniProt/Swiss-Prot:P49427

Protein names

UBC3, UBCH3, UBE2R1, E2-CDC34

Protein function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes ‘Lys-48’-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions ‘Lys-21’ and/or ‘Lys-22’ with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.

Protein tissue specificity

Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription.

Protein sequence and domain

The C-terminal acidic tail is required for nuclear localization and is involved in the binding to SCF E3 ligase complexes, and more specifically with the CUL1 subunit.Belongs to the ubiquitin-conjugating enzyme family.

Protein post-translational modifications

Autoubiquitinated. Autoubiquitination is promoted by the human herpes virus 1 protein ICP0 and leads to degradation by the Ubiquitin-proteasomal pathway.; Phosphorylated by CK2. Phosphorylation of the C-terminal tail by CK2 controles the nuclear localization.

Protein cellular localization

Cytoplasm. Nucleus. Note: The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.

Background

The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication.

Research area

All research areas>Cell Cycle Proteins>Cdc
(View all antibody categories related to Cell Cycle Proteins)

Note

AntibodyPlus can customize CDC34 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!