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Rabbit polyclonal CDC42 antibody (A1188)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC
WB 1:500 – 1:2000 IHC 1:50 – 1:200
Recommended protocols: check protocols
Click or hover above images to see image description for Rabbit polyclonal CDC42 antibody.
Check alternative names for the antibodyExpand
CDC42 antibody,CDC42Hs antibody,G25K antibody
CDC42 antibody|CDC42_HUMAN antibody|CDC42Hs antibody|Cell division control protein 42 homolog antibody|Cell division cycle 42 (GTP binding protein 25kDa) antibody|Cell division cycle 42 antibody|dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody|dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody|G25K antibody|G25K GTP-binding protein antibody|Growth regulating protein antibody|GTP binding protein 25kDa antibody|Small GTP binding protein CDC42 antibody|TKS antibody|Anti-CDC42 antibody (ab64533)
SCBT cat No: sc-8401|sc-6083|sc-87|
Rabbit polyclonal CDC42 antibody
|Catalogue No.|| |
Human, Mouse, Rat
Recombinant protein of human CDC42
|Recommended dilution|| |
WB 1:500 – 1:2000
|Molecular weight|| |
Predicted: 21kDa/Observed: Refer to Figures
CDC42 antibody was tube-contained.
CDC42 antibody was purified using affinity purification.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
CDC42 antibody,CDC42Hs antibody,G25K antibody
|Database links|| |
|Protein names|| |
|Protein function|| |
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration.
|Protein sequence and domain|| |
Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
|Protein post-translational modifications|| |
(Microbial infection) AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.; Phosphorylated by SRC in an EGF-dependent manner, this stimulates the binding of the Rho-GDP dissociation inhibitor RhoGDI.; (Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of CDC42 and leads to actin disassembly.
|Protein cellular localization|| |
Cell membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Midbody. Note: Localizes to spindle during prometaphase cells. Moves to the central spindle as cells progressed through anaphase to telophase. Localizes at the end of cytokinesis in the intercellular bridge formed between two daughter cells. Its localization is regulated by the activities of guanine nucleotide exchange factor ECT2 and GTPase activating protein RACGAP1. Colocalizes with NEK6 in the centrosome.
Rac and Cdc42 are members of the Rho-GTPase family. In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac1 and Cdc42, the most widely studied of this group, are ubiquitously expressed. Rac2 is expressed in cells of hematopoietic origin, and Rac3, while highly expressed in brain, is also found in many other tissues. Rac and Cdc42 play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, cell growth and development (1). GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the protein’s intrinsic GTPase activity, rendering it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level of regulation is achieved through the binding of RhoGDI, a guanine nucleotide dissociation inhibitor, which retains Rho family GTPases, including Rac and Cdc42, in their inactive GDP-bound state (2,3).
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|Product type|| |
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