Rabbit polyclonal CDH1 antibody (A3044)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IP
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal CDH1 antibody (A3044)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IP

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IP 1:20 – 1:50

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

CDH1 antibody, Arc-1 antibody,CD324 antibody,CDHE antibody, ECAD antibody,LCAM antibody, UVO antibody,E-Cad/CTF3 antibody,Uvomorulin antibody, E-Cadherin antibody
Arc 1 antibody|CADH1_HUMAN antibody|Cadherin 1 antibody|cadherin 1 type 1 E-cadherin antibody|Cadherin1 antibody|CAM 120/80 antibody|CD 324 antibody|CD324 antibody|CD324 antigen antibody|cdh1 antibody|CDHE antibody|E-Cad/CTF3 antibody|E-cadherin antibody|ECAD antibody|Epithelial cadherin antibody|epithelial calcium dependant adhesion protein antibody|LCAM antibody|Liver cell adhesion molecule antibody|UVO antibody|Uvomorulin antibody|Anti-E Cadherin antibody [HECD-1] (ab1416)

SCBT cat No: sc-71009|sc-71008|sc-71007|sc-52327|sc-21791|sc-52328|sc-59778|sc-8426|sc-7870|sc-31021|sc-59905|sc-1500|sc-31020|sc-59780|sc-56527|sc-137991|sc-376017|

Name

Rabbit polyclonal CDH1 antibody

Catalogue No.

A3044

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human CDH1

Host

Rabbit

Applications

WB, IHC, IP

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IP 1:20 – 1:50

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 135kDa/Observed: Refer to Figures

Formulation

CDH1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

CDH1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CDH1 antibody, Arc-1 antibody,CD324 antibody,CDHE antibody, ECAD antibody,LCAM antibody, UVO antibody,E-Cad/CTF3 antibody,Uvomorulin antibody, E-Cadherin antibody

Database links

Human UniProt/Swiss-Prot:P12830

Protein names

CDH1, Arc-1,CD324,CDHE, ECAD,LCAM, UVO,E-Cad/CTF3,Uvomorulin, E-Cadherin

Protein function

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.; E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Protein tissue specificity

Non-neural epithelial tissues.

Protein sequence and domain

Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.Contains 5 cadherin domains.

Protein post-translational modifications

During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.; N-glycosylation at Asn-637 is essential for expression, folding and trafficking.; Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754.

Protein cellular localization

Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Note: Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq, Jul 2008]

Research area

All research areas>Cell Adhesion Proteins>Cadherin
(View all antibody categories related to Cell Adhesion Proteins)

Note

AntibodyPlus can customize CDH1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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