Rabbit Polyclonal Cdk4 antibody (STJ96563)

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Reactivity: Human, Mouse, Rat
Applications: WB, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Cdk4 antibody (STJ96563)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC-P 1:100-300; ELISA 1:20000;

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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CDK4 antibody,|Cdk 4 antibody|cdk4 antibody|CDK4 protein antibody|CDK4_HUMAN antibody|Cell division kinase 4 antibody|Cell division protein kinase 4 antibody|CMM 3 antibody|CMM3 antibody|Crk3 antibody|Cyclin dependent kinase 4 antibody|Cyclin-dependent kinase 4 antibody|Melanoma cutaneous malignant 3 antibody|MGC14458 antibody|p34 cdk4 antibody|PSK J3 antibody|PSK-J3 antibody|Anti-Cdk4 antibody [EPR4513] (ab108357) KO Validated
SCBT cat No: sc-70832|sc-70831|sc-136241|sc-166373|sc-260|sc-53636|sc-56277|sc-23896|sc-393653|sc-601|sc-749|sc-56361|

Name

Cdk4 Polyclonal Antibody

Catalogue No.

STJ96563

Reactivity

Human, Mouse, Rat

Specificity

Cdk4 Polyclonal Antibody detects endogenous levels of Cdk4 protein.

Immunogen

Synthesized peptide derived from Cdk4 at AA range 1-50

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC-P 1:100-300; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

180/35 kDa

Formulation

Cdk4 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Cdk4 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Cyclin-dependent kinase 4 antibody, Cell division protein kinase 4 antibody, PSK-J3 antibody

Database links

Human UniProt/Swiss-Prot:P11802;Mouse UniPort/Swiss-Prot: P30285;Rat UniProt/Swiss-Port: P35426;Human Entrez Gene: 1019;Mouse Entrez Gene: 12567;Rat Entrez Gene: Rn.6115

Protein names

Cyclin-dependent kinase 4 , Cell division protein kinase 4 , PSK-J3

Protein function

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. / ATP + a protein = ADP + a phosphoprotein. / Both phosphorylation at Thr-172 and binding of a D-type cyclin are necessary for enzymatic activity. Full activation of the cyclin-D-CDK4 complex appears to require other factors such as recruitment of the substrate via a substrate recruitment motif, and/or formation of the CDKN1B ternary complex. Inhibited by INK4 family members. In resting cells, the non-tyrosine-phosphorylated form of CDKN1B prevents phosphorylation at Thr-172 and inactivation, while, in proliferating cells, tyrosine phosphorylation of CDKN1B allows phosphorylation of Thr-172 of CDK4 and subsequennt activation.

Involvement in disease

Melanoma, cutaneous malignant 3 (CMM3) [MIM:609048]: A malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites. . Note: Disease susceptibility is associated with variations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. / Contains 1 protein kinase domain.

Protein post-translational modifications

Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.

Protein cellular localization

Cytoplasm / Nucleus / Membrane

Research area

All research areas>Cell Cycle Proteins>Cdk
(View all antibody categories related to Cell Cycle Proteins)

Note

AntibodyPlus can customize Cdk4 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human stomach tissue

1: Cdk4 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat lung tissue

1: Cdk4 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.


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