Rabbit polyclonal CDK4 antibody (A0366)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal CDK4 antibody (A0366)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:20 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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CDK4 antibody,CMM3 antibody,MGC14458 antibody,PSK-J3 antibody
Cdk 4 antibody|cdk4 antibody|CDK4 protein antibody|CDK4_HUMAN antibody|Cell division kinase 4 antibody|Cell division protein kinase 4 antibody|CMM 3 antibody|CMM3 antibody|Crk3 antibody|Cyclin dependent kinase 4 antibody|Cyclin-dependent kinase 4 antibody|Melanoma cutaneous malignant 3 antibody|MGC14458 antibody|p34 cdk4 antibody|PSK J3 antibody|PSK-J3 antibody|Anti-Cdk4 antibody [EPR4513] (ab108357) KO Validated

SCBT cat No: sc-70832|sc-70831|sc-136241|sc-166373|sc-260|sc-53636|sc-56277|sc-23896|sc-393653|sc-601|sc-749|sc-56361|

Name

Rabbit polyclonal CDK4 antibody

Catalogue No.

A0366

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human CDK4

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:20 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 34kDa/Observed: Refer to Figures

Formulation

CDK4 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

CDK4 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CDK4 antibody,CMM3 antibody,MGC14458 antibody,PSK-J3 antibody

Database links

Human UniProt/Swiss-Prot:P11802

Protein names

CDK4,CMM3,MGC14458,PSK-J3

Protein function

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Protein sequence and domain

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.; Contains 1 protein kinase domain.

Protein post-translational modifications

Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.

Protein cellular localization

Cytoplasm. Nucleus. Membrane. Note: Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.

Background

Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase (2). The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle (4).

Research area

All research areas>Cell Cycle Proteins>Cdk
(View all antibody categories related to Cell Cycle Proteins)

Note

AntibodyPlus can customize CDK4 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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