Rabbit polyclonal CDKN1B antibody (A0290)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal CDKN1B antibody (A0290)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:200 – 1:500 IHC 1:50 – 1:200 IF 1:20 – 1:50

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal CDKN1B antibody.

Alternative names:

Check alternative names for the antibody

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CDKN1B antibody,CDKN4 antibody,KIP1 antibody,MEN1B antibody,MEN4 antibody,P27KIP1 antibody
AA408329 antibody|AI843786 antibody|Cdki1b antibody|CDKN 1B antibody|CDKN 4 antibody|CDKN1B antibody|CDKN4 antibody|CDN1B_HUMAN antibody|Cyclin Dependent Kinase Inhibitor 1B antibody|Cyclin dependent kinase inhibitor p27 antibody|Cyclin-dependent kinase inhibitor 1B (p27, Kip1) antibody|Cyclin-dependent kinase inhibitor 1B antibody|Cyclin-dependent kinase inhibitor p27 antibody|Cyclin-dependent kinase inhibitor p27 Kip1 antibody|KIP 1 antibody|KIP1 antibody|MEN1B antibody|MEN4 antibody|OTTHUMP00000195098 antibody|OTTHUMP00000195099 antibody|p27 antibody|p27 Kip1 antibody|P27-like cyclin-dependent kinase inhibitor antibody|p27Kip1 antibody|Anti-p27 KIP 1 antibody [Y236] (ab32034)

SCBT cat No: sc-393380|sc-528|sc-393305|sc-56338|sc-1641|sc-776|sc-527|sc-56454|sc-53906|sc-53871|sc-6359|sc-9962|

Name

Rabbit polyclonal CDKN1B antibody

Catalogue No.

A0290

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human CDKN1B

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:200 – 1:500
IHC 1:50 – 1:200
IF 1:20 – 1:50

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 27kDa/Observed: Refer to Figures

Formulation

CDKN1B antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

CDKN1B antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CDKN1B antibody,CDKN4 antibody,KIP1 antibody,MEN1B antibody,MEN4 antibody,P27KIP1 antibody

Database links

Human UniProt/Swiss-Prot:P46527

Protein names

CDKN1B,CDKN4,KIP1,MEN1B,MEN4,P27KIP1

Protein function

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Protein tissue specificity

Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

Protein sequence and domain

A peptide sequence containing only AA 28-79 retains substantial Kip1 cyclin A/CDK2 inhibitory activity.Belongs to the CDI family.

Protein post-translational modifications

Phosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK1 and CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF.; Ubiquitinated; in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated; by a TRIM21-containing SCF(SKP2)-like complex; leads to its degradation.; Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation (By similarity).

Protein cellular localization

Nucleus. Cytoplasm. Endosome . Note: Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity).

Background

CDKN1B Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of CDKN1B are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of CDKN1B can be induced by treatment with interleukin 2 or other mitogens; this involves phosphorylation of CDKN1B and its degradation by the ubiquitin-proteasome pathway (1-4).

Research area

All research areas>Cell Cycle Proteins>p27
(View all antibody categories related to Cell Cycle Proteins)

Note

AntibodyPlus can customize CDKN1B antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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