Rabbit polyclonal CFLAR antibody (A2555)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Rabbit polyclonal CFLAR antibody (A2555)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal CFLAR antibody.

Alternative names:

Check alternative names for the antibody

Expand

CASH antibody, CASP8AP1 antibody, CLARP antibody, Casper antibody, FLAME antibody, FLAME-1 antibody, FLAME1 antibody, FLIP antibody, I-FLICE antibody, MRIT antibody, c-FLIP antibody, c-FLIPL antibody, c-FLIPR antibody, c-FLIPS antibody
c FLIP antibody|MRIT antibody|OTTHUMP00000163715 antibody|OTTHUMP00000206475 antibody|OTTHUMP00000206476 antibody|OTTHUMP00000206478 antibody|OTTHUMP00000206479 antibody|OTTHUMP00000206480 antibody|OTTHUMP00000206482 antibody|OTTHUMP00000207360 antibody|Usurpin antibody|Usurpin beta antibody|Anti-FLIP antibody (ab8421)

SCBT cat No: sc-7108|sc-8346|sc-7109|sc-8347|

Name

Rabbit polyclonal CFLAR antibody

Catalogue No.

A2555

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human CFLAR

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 52kDa/Observed: Refer to Figures

Formulation

CFLAR antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

CFLAR antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CASH antibody, CASP8AP1 antibody, CLARP antibody, Casper antibody, FLAME antibody, FLAME-1 antibody, FLAME1 antibody, FLIP antibody, I-FLICE antibody, MRIT antibody, c-FLIP antibody, c-FLIPL antibody, c-FLIPR antibody, c-FLIPS antibody

Database links

Human UniProt/Swiss-Prot:O15519

Protein names

CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS

Protein function

Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.

Protein tissue specificity

Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.

Protein sequence and domain

The caspase domain lacks the active sites residues involved in catalysis.Belongs to the peptidase C14A family.; Contains 2 DED (death effector) domains.

Protein post-translational modifications

Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.

Background

Cellular FLIP (FLICE inhibitory protein) is a regulator of apoptosis that has various names, such as c-FLIP (1), Casper (2), CLARP (3), FLAME (4), I-FLICE (5), MRIT (6), CASH (7), and Usurpin (8). FLIP is expressed as two alternative splice isoforms, FLIP short (FLIPS) and FLIP long (FLIPL). FLIPS contains two death effector domains (DEDs) like those found on the death receptor adaptor protein FADD and the pro-domain of caspase-8. FLIPL shares significant homology with caspase-8 (FLICE), and contains an additional death effector domain, but FLIPL lacks the catalytic active site of the caspases and does not have protease activity. Both FLIP isoforms have been reported to interact with FADD and pro-caspase-8. The role of FLIP in apoptosis is controversial as some research studies have reported it to be anti-apoptotic, while others claim that it is pro-apoptotic. Overexpression of FLIPL can lead to caspase-8 heterodimers that produce an active protease, resulting in apoptosis. However, at physiological levels, it is thought that the binding of FLIP to the DED of FADD results in inhibition of caspase-8 processing. Reduction of FLIP by siRNA or gene targeting sensitizes cells to death receptor-mediated apoptosis. FLIP has also been implicated in the resistance of cancer cells to apoptosis and is upregulated in some cancer types including Hodgkin’s lymphoma and ovarian and colon carcinomas (9).

Research area

All research areas>Tumor Suppressor/Apoptosis>FLIP
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize CFLAR antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!