Rabbit polyclonal CHAF1B antibody (A6259)

$69.00$259.00

Reactivity: Human,Mouse
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal CHAF1B antibody (A6259)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:20 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal CHAF1B antibody.

Alternative names:

Check alternative names for the antibody

Expand

CAF1 antibody, MPP7 antibody, CAF-1 antibody, CAF1A antibody, CAF1P60 antibody, CAF-IP60 antibody, MPHOSPH7 antibody
CAF 1 subunit B antibody|CAF 1A antibody|CAF I 60 kDa subunit antibody|CAF IP60 antibody|CAF-1 antibody|CAF-1 subunit B antibody|CAF-I 60 kDa subunit antibody|CAF-I p60 antibody|CAF1 antibody|CAF1A antibody|CAF1B_HUMAN antibody|CAF1P60 antibody|CHAF 1B antibody|CHAF1B antibody|Chromatin assembly factor 1 subunit B antibody|Chromatin assembly factor I antibody|Chromatin assembly factor I p60 subunit antibody|Human chromatin assembly factor-I p60 subunit antibody|M phase phosphoprotein 7 antibody|M-phase phosphoprotein 7 antibody|MPHOSPH7 antibody|MPP 7 antibody|MPP7 antibody|p60 subunit antibody|Anti-p60 CAF1 antibody [EPR6105] (ab109442)

SCBT cat No: sc-393662|sc-10982|sc-367905|sc-56646|

Name

Rabbit polyclonal CHAF1B antibody

Catalogue No.

A6259

Reactivity

Human, Mouse

Immunogen

Recombinant protein of human CHAF1B

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:20 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 61kDa/Observed: Refer to Figures

Formulation

CHAF1B antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

CHAF1B antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CAF1 antibody, MPP7 antibody, CAF-1 antibody, CAF1A antibody, CAF1P60 antibody, CAF-IP60 antibody, MPHOSPH7 antibody

Database links

Human UniProt/Swiss-Prot:Q13112

Protein names

CAF1, MPP7, CAF-1, CAF1A, CAF1P60, CAF-IP60, MPHOSPH7

Protein function

Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.

Protein sequence and domain

Belongs to the WD repeat HIR1 family.; Contains 7 WD repeats.

Protein post-translational modifications

Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.

Protein cellular localization

Nucleus . Cytoplasm . Note: DNA replication foci. Cytoplasmic in M phase.

Background

Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.

Research area

All research areas>Transcription Regulators>CAF-1
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize CHAF1B antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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