Rabbit polyclonal CHEK2 antibody (A2145)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,ICC,IF,IP
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal CHEK2 antibody (A2145)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,ICC,IF,IP

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 ICC 1:50 – 1:200 IF 1:50 – 1:200 IP 1:20 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal CHEK2 antibody.

Alternative names:

Check alternative names for the antibody

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CDS1 antibody, CHK2 antibody, HuCds1 antibody, LFS2 antibody, PP1425 antibody, RAD53 antibody
CDS 1 antibody|Cds1 antibody|Cds1 homolog antibody|Checkpoint kinase 2 antibody|Checkpoint like protein CHK2 antibody|CHEK 2 antibody|Chek2 antibody|Chk 2 antibody|CHK2 checkpoint homolog (S. pombe) antibody|CHK2 checkpoint homolog antibody|CHK2_HUMAN antibody|hCds1 antibody|HuCds 1 antibody|LFS 2 antibody|LFS2 antibody|PP1425 antibody|RAD 53 antibody|RAD53 antibody|Rad53 homolog antibody|Serine/threonine protein kinase Chk2 antibody|Serine/threonine-protein kinase Chk2 antibody|Anti-Chk2 antibody [EPR4325] (ab109413) KO Validated

SCBT cat No: sc-136251|sc-17747|sc-5278|sc-17748|sc-56296|sc-56297|sc-9064|sc-8812|sc-293293|sc-34986|

Name

Rabbit polyclonal CHEK2 antibody

Catalogue No.

A2145

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human CHEK2

Host

Rabbit

Applications

WB, IHC, ICC, IF, IP

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
ICC 1:50 – 1:200
IF 1:50 – 1:200
IP 1:20 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 61kDa/Observed: Refer to Figures

Formulation

CHEK2 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

CHEK2 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CDS1 antibody, CHK2 antibody, HuCds1 antibody, LFS2 antibody, PP1425 antibody, RAD53 antibody

Database links

Human UniProt/Swiss-Prot:O96017

Protein names

CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53

Protein function

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at ‘Ser-20’ by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition .

Protein tissue specificity

High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.

Protein sequence and domain

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.; Contains 1 FHA domain.; Contains 1 protein kinase domain.

Protein post-translational modifications

Phosphorylated. Phosphorylated at Ser-73 by PLK3 in response to DNA damage, promoting phosphorylation at Thr-68 by ATM and the G2/M transition checkpoint. Phosphorylation at Thr-68 induces homodimerization. Autophosphorylates at Thr-383 and Thr-387 in the T-loop/activation segment upon dimerization to become fully active and phosphorylate its substrates like for instance CDC25C. DNA damage-induced autophosphorylation at Ser-379 induces CUL1-mediated ubiquitination and regulates the pro-apoptotic function. Phosphorylation at Ser-456 also regulates ubiquitination. Phosphorylated by PLK4.; Ubiquitinated. CUL1-mediated ubiquitination regulates the pro-apoptotic function. Ubiquitination may also regulate protein stability. Ubiquitinated by RNF8 via ‘Lys-48’-linked ubiquitination.

Protein cellular localization

Isoform 2: Nucleus. Note: Isoform 10 is present throughout the cell.; Isoform 4: Nucleus.; Isoform 7: Nucleus.; Isoform 9: Nucleus.; Isoform 12: Nucleus.; Nucleus, PML body. Nucleus, nucleoplasm. Note: Recruited into PML bodies together with TP53.

Background

Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50, and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5). After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 at residues Thr383 and Thr387 in the activation loop of the kinase domain (8).

Research area

All research areas>Cell Cycle Proteins>Chk
(View all antibody categories related to Cell Cycle Proteins)

Note

AntibodyPlus can customize CHEK2 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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