Rabbit polyclonal CHMP4B antibody (A7402)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal CHMP4B antibody (A7402)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

SNF7 antibody, CTPP3 antibody, Shax1 antibody, CHMP4A antibody, SNF7-2 antibody, VPS32B antibody, CTRCT31 antibody, Vps32-2 antibody, C20orf178 antibody, dJ553F4.4 antibody
Charged multivesicular body protein 4b antibody|CHM4B_HUMAN antibody|chmp4b antibody|Chromatin-modifying protein 4b antibody|hSnf7-2 antibody|hVps32-2 antibody|SNF7 homolog associated with Alix 1 antibody|SNF7-2 antibody|Vacuolar protein sorting-associated protein 32-2 antibody|Vps32-2 antibody|Anti-CHMP4B antibody (ab105767)

SCBT cat No: sc-82556|sc-134946|sc-82557|

Name

Rabbit polyclonal CHMP4B antibody

Catalogue No.

A7402

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human CHMP4B

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 25kDa/Observed: Refer to figures

Formulation

CHMP4B antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

CHMP4B antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

SNF7 antibody, CTPP3 antibody, Shax1 antibody, CHMP4A antibody, SNF7-2 antibody, VPS32B antibody, CTRCT31 antibody, Vps32-2 antibody, C20orf178 antibody, dJ553F4.4 antibody

Database links

Human UniProt/Swiss-Prot:Q9H444

Protein names

SNF7, CTPP3, Shax1, CHMP4A, SNF7-2, VPS32B, CTRCT31, Vps32-2, C20orf178, dJ553F4.4

Protein function

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released . The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis . Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase . Plays a role in the endosomal sorting pathway. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4B filaments can promote or stabilize negative curvature and outward budding. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan .; (Microbial infection) The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the budding of enveloped viruses (HIV-1 and other lentiviruses). Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release.

Protein tissue specificity

Widely expressed. Expressed at higher level in heart and skeletal muscle. Also expressed in brain, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes.

Protein sequence and domain

The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.Belongs to the SNF7 family.

Protein post-translational modifications

ISGylated. Isgylation weakens its interaction with VPS4A.

Protein cellular localization

Cytoplasm, cytosol . Late endosome membrane ; Peripheral membrane protein . Midbody . Nucleus envelope . Note: Recruited to the nuclear envelope by CHMP7 during late anaphase .

Background

This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies. Mutations in this gene result in autosomal dominant posterior polar cataracts.

Note

AntibodyPlus can customize CHMP4B antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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