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Rabbit Polyclonal Cleaved-Caspase-4/5 p20 (D270/D311) antibody (STJ90028)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:40000;
Recommended protocols: check protocols
Click or hover above images to see image description for Cleaved-Caspase-4/5 p20 (D270/D311) Polyclonal Antibody.
Check alternative names for the antibodyExpand
CASP4 antibody, ICH2 antibody,|Apoptotic cysteine protease Mih1/TX antibody|Caspase-4 subunit 2 antibody|Caspase4 antibody|ICE(rel)-II antibody|ICE(rel)II antibody|ICEREL II antibody|ICH2 antibody|Mih1/TX antibody|Protease ICH-2 antibody|Protease TX antibody|TX antibody|Anti-Caspase 4 antibody (ab25898)
SCBT cat No: sc-56038|sc-15881|sc-15882|sc-28231|sc-374615|sc-28230|
Cleaved-Caspase-4/5 p20 (D270/D311) Polyclonal Antibody
|Catalogue No.|| |
Cleaved-Caspase-4/5 p20 (D270/D311) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-4/5 p20 protein resulting from cleavage adjacent to D270/D311.
Synthesized peptide derived from Cleaved-Caspase-4/5 p20 (D270/D311) at AA range 190-270
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:40000;
|Molecular weight|| |
Cleaved-Caspase-4/5 p20 (D270/D311) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Cleaved-Caspase-4/5 p20 (D270/D311) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Caspase-4 antibody, CASP-4 antibody, ICE and Ced-3 homolog 2 antibody, ICH-2 antibody, ICEantibody, rel antibody,-II antibody, Mih1 antibody, Protease TX antibody
|Protein names|| |
Caspase-4 , CASP-4 , ICE and Ced-3 homolog 2 , ICH-2 , ICE, rel ,-II , Mih1 , Protease TX
|Protein function|| |
Inflammatory caspase . Essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS . Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, and IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators . Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection . In later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which ultimately results in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation. Involved in LPS-induced IL6 secretion; this activity may not require caspase enzymatic activity . Involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP peptides found Alzheimer’s patient brains . Activated by direct binding to LPS without the need of an upstream sensor . Does not directly process IL1B . / Strict requirement for Asp at the P1 position. It has a preferred cleavage sequence of Tyr-Val-Ala-Asp-, – but also cleaves at Asp-Glu-Val-Asp-, -. / Activated by homooligomerization induced by direct binding to cytosolic LPS, in a TLR4-independent manner . / (Microbial infection) Inhibited by the effector protein NleF produced by pathogenic E.coli; this inhibits apoptosis . / Values obtained using the partial C-terminal enzyme sequence of 105-377. / 681 µM for synthetic peptide acetyl-YVAD-p-nitroanilide
|Protein tissue specificity|| |
Widely expressed, including in keratinocytes and colonic and small intestinal epithelial cells (at protein level). Not detected in brain.
|Protein sequence and domain|| |
The CARD domain mediates LPS recognition and homooligomerization. / Belongs to the peptidase C14A family. / Contains 1 CARD domain.
|Protein post-translational modifications|| |
In response to activation signals, including endoplasmic reticulum stress or treatment with amyloid beta A4 protein fragments (such as beta-amyloid protein 40), undergoes autoproteolytic cleavage.
|Protein cellular localization|| |
Cytoplasm > cytosol / Endoplasmic reticulum membrane / Peripheral membrane protein / Cytoplasmic side / Mitochondrion / Inflammasome / Secreted
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St John’s Laboratory Ltd.
|Product type|| |
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