Rabbit Polyclonal Cleaved-Caspase-9 p35 (D315) antibody (STJ90013)

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Reactivity: Human
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Cleaved-Caspase-9 p35 (D315) antibody (STJ90013)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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CASP9 antibody, MCH6 antibody,|APAF-3 antibody|Apoptotic protease Mch-6 antibody|Caspase 9 Dominant Negative antibody|Caspase 9c antibody|Caspase-9 antibody|Caspase-9 subunit p10 antibody|ICE LAP6 antibody|ICE like apoptotic protease 6 antibody|ICE-LAP6 antibody|ICE-like apoptotic protease 6 antibody|MCH6 antibody|PPP1R56 antibody|protein phosphatase 1, regulatory subunit 56 antibody|RNCASP9 antibody|Anti-Caspase-9 antibody [E23] (ab32539)
SCBT cat No: sc-81650|sc-56073|sc-70505|sc-70506|sc-70507|sc-56076|sc-81663|sc-81589|sc-73548|sc-56077|sc-8297|sc-17784|sc-7885|sc-133109|sc-8355|sc-374489|sc-373777|

Name

Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody

Catalogue No.

STJ90013

Reactivity

Human

Specificity

Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-9 p35 protein resulting from cleavage adjacent to D315.

Immunogen

Synthesized peptide derived from Cleaved-Caspase-9 p35 (D315) at AA range 240-320

Host

Rabbit

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

35 kDa

Formulation

Cleaved-Caspase-9 p35 (D315) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Cleaved-Caspase-9 p35 (D315) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Caspase-9 antibody, CASP-9 antibody, Apoptotic protease Mch-6 antibody, Apoptotic protease-activating factor 3 antibody, APAF-3 antibody, ICE-like apoptotic protease 6 antibody, ICE-LAP6 antibody

Database links

Human UniProt/Swiss-Prot:P55211;Mouse UniPort/Swiss-Prot: Q8C3Q9;Rat UniProt/Swiss-Port: Q9JHK1;Human Entrez Gene: 842;Mouse Entrez Gene: 12371;Rat Entrez Gene: Rn.32199

Protein names

Caspase-9 , CASP-9 , Apoptotic protease Mch-6 , Apoptotic protease-activating factor 3 , APAF-3 , ICE-like apoptotic protease 6 , ICE-LAP6

Protein function

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). / Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. / Strict requirement for an Asp residue at position P1 and with a marked preference for His at position P2. It has a preferred cleavage sequence of Leu-Gly-His-Asp-, -Xaa. / Inhibited by the effector protein NleF that is produced by pathogenic E.coli; this inhibits apoptosis.

Protein tissue specificity

Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.

Protein sequence and domain

Belongs to the peptidase C14A family. / Contains 1 CARD domain.

Protein post-translational modifications

Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. / Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage.

Research area

All research areas>Tumor Suppressor/Apoptosis>Caspase
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize Cleaved-Caspase-9 p35 (D315) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human breast tissue

1: Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human breast tissue

1: Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human liver tissue

1: Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human lung tissue

1: Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human lung cancer tissue

1: Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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