Rabbit Polyclonal COL1A2 antibody (STJ92383)

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Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal COL1A2 antibody (STJ92383)

Supplier: St John’s Laboratory Ltd.

Recommended applications: IHC, IF, ELISA

Recommended dilution: IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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COL1A2 antibody,|Alpha 1 type I collagen antibody|Alpha 2 type I collagen antibody|alpha 2 type I procollagen antibody|alpha 2(I) procollagen antibody|alpha 2(I)-collagen antibody|Alpha-1 type I collagen antibody|alpha1(I) procollagen antibody|CO1A1_HUMAN antibody|COL1A1 antibody|COL1A2 antibody|collagen alpha 1 chain type I antibody|Collagen alpha-1(I) chain antibody|collagen alpha-1(I) chain preproprotein antibody|Collagen I alpha 1 polypeptide antibody|Collagen I alpha 2 polypeptide antibody|collagen of skin, tendon and bone, alpha-1 chain antibody|collagen of skin, tendon and bone, alpha-2 chain antibody|Collagen type I alpha 1 antibody|Collagen type I alpha 2 antibody|EDSC antibody|OI1 antibody|OI2 antibody|OI3 antibody|OI4 antibody|pro-alpha-1 collagen type 1 antibody|type I proalpha 1 antibody|type I procollagen alpha 1 chain antibody|Type I procollagen antibody|Anti-Collagen I antibody (ab34710)
SCBT cat No: sc-30136|

Name

COL1A2 Polyclonal Antibody

Catalogue No.

STJ92383

Reactivity

Human, Mouse, Rat

Specificity

COL1A2 Polyclonal Antibody detects endogenous levels of COL1A2 protein.

Immunogen

Synthesized peptide derived from COL1A2 at AA range 1-80

Host

Rabbit

Applications

IHC, IF, ELISA

Recommended dilution

IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

92 kDa

Formulation

COL1A2 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

COL1A2 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Collagen alpha-2antibody, I antibody, chain antibody, Alpha-2 type I collagen antibody

Database links

Human UniProt/Swiss-Prot:P08123;Mouse UniPort/Swiss-Prot: Q01149;Rat UniProt/Swiss-Port: P02466;Human Entrez Gene: 1278;Mouse Entrez Gene: 12843;Rat Entrez Gene: Rn.107239

Protein names

Collagen alpha-2, I , chain , Alpha-2 type I collagen

Protein function

Type I collagen is a member of group I collagen (fibrillar forming collagen).

Protein tissue specificity

Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

Involvement in disease

Ehlers-Danlos syndrome 7B (EDS7B) [MIM:130060]: A connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. Marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Osteogenesis imperfecta 1 (OI1) [MIM:166200]: An autosomal dominant form of osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma. Disease severity ranges from very mild forms without fractures to intrauterine fractures and perinatal lethality. Extraskeletal manifestations, which affect a variable number of patients, are dentinogenesis imperfecta, hearing loss, and blue sclerae. OI1 is a non-deforming form with normal height or mild short stature, and no dentinogenesis imperfecta. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Osteogenesis imperfecta 2 (OI2) [MIM:166210]: An autosomal dominant form of osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma. Disease severity ranges from very mild forms without fractures to intrauterine fractures and perinatal lethality. Extraskeletal manifestations, which affect a variable number of patients, are dentinogenesis imperfecta, hearing loss, and blue sclerae. OI2 is characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Ehlers-Danlos syndrome, autosomal recessive, cardiac valvular form (EDSCV) [MIM:225320]: A connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. In addition to joint laxity, skin hyperextensibility and friability, and abnormal scar formation, patients have mitral valve prolapse and insufficiency, mitral regurgitation, and aortic insufficiency. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Osteogenesis imperfecta 3 (OI3) [MIM:259420]: An autosomal dominant form of osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma. Disease severity ranges from very mild forms without fractures to intrauterine fractures and perinatal lethality. Extraskeletal manifestations, which affect a variable number of patients, are dentinogenesis imperfecta, hearing loss, and blue sclerae. OI3 is characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera and dentinogenesis imperfecta. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Osteogenesis imperfecta 4 (OI4) [MIM:166220]: An autosomal dominant form of osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma. Disease severity ranges from very mild forms without fractures to intrauterine fractures and perinatal lethality. Extraskeletal manifestations, which affect a variable number of patients, are dentinogenesis imperfecta, hearing loss, and blue sclerae. OI4 is characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Note: A chromosomal aberration involving COL1A2 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. Translocation t(7;8)(p22;q13) with PLAG1.

Protein sequence and domain

The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function (By similarity). / Belongs to the fibrillar collagen family. / Contains 1 fibrillar collagen NC1 domain.

Protein post-translational modifications

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

Protein cellular localization

Secreted > extracellular space > extracellular matrix

Research area

All research areas>Cell Adhesion Proteins>Collagen
(View all antibody categories related to Cell Adhesion Proteins)

Note

AntibodyPlus can customize COL1A2 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human stomach tissue

1: COL1A2 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human stomach tissue

1: COL1A2 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat lung tissue

1: COL1A2 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat lung tissue

1: COL1A2 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Rat lung tissue

1: COL1A2 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse liver tissue

1: COL1A2 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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