Rabbit polyclonal CRK antibody (A1604)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal CRK antibody (A1604)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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p38 antibody, CRKII antibody
Adapter molecule crk antibody|Avian sarcoma virus CT10 (v crk) oncogene homolog antibody|CRK antibody|CRK isoform 2 antibody|CRK isoform II antibody|CRK_HUMAN antibody|CRKII antibody|FLJ38130 antibody|OTTHUMP00000115366 antibody|OTTHUMP00000198330 antibody|p38 antibody|Proto oncogene C crk antibody|Proto-oncogene C-crk antibody|v crk avian sarcoma virus CT10 oncogene homolog antibody|v crk sarcoma virus CT10 oncogene homolog antibody|v crk sarcoma virus CT10 oncogene homolog (avian) antibody|Anti-Crk p38 antibody [EPR4984(2)] (ab133581)

SCBT cat No: sc-393160|sc-23775|sc-17989|sc-81234|sc-390132|sc-289|sc-9004|

Name

Rabbit polyclonal CRK antibody

Catalogue No.

A1604

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human CRK

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 34kDa/Observed: Refer to figures

Formulation

CRK antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

CRK antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

p38 antibody, CRKII antibody

Database links

Human UniProt/Swiss-Prot:P46108

Protein names

p38, CRKII

Protein function

Isoform Crk-II: Regulates cell adhesion, spreading and migration. Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.

Protein sequence and domain

The C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation.; The SH2 domain mediates interaction with tyrosine phosphorylated proteins. Mediates interaction with SHB.Belongs to the CRK family.; Contains 1 SH2 domain.; Contains 2 SH3 domains.

Protein post-translational modifications

Phosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form. Isoform Crk-II is phosphorylated by KIT.; Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway.; Proline isomerization at Pro-237 by PPIA acts as a switch between two conformations: an autoinhibitory conformation in the cis form, where the tandem SH3 domains interact intramolecularly, and an activated conformation in the trans form.

Protein cellular localization

Cytoplasm . Cell membrane . Note: Translocated to the plasma membrane upon cell adhesion.

Background

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.

Research area

All research areas>Signaling Intermediates>Crk
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus can customize CRK antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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