Rabbit polyclonal CRYAA antibody (A5725)

$69.00$259.00

Reactivity: Human,Mouse,Rat,Zebrafish
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal CRYAA antibody (A5725)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal CRYAA antibody.

Alternative names:

Check alternative names for the antibody

Expand

CRYA1 antibody, HSPB4 antibody, CTRCT9 antibody
Acry 1 antibody|Alpha crystallin A chain antibody|Alpha-crystallin A chain antibody|CRYA 1 antibody|CRYA1 antibody|CRYAA antibody|CRYAA_HUMAN antibody|Crystallin alpha 1 antibody|Crystallin alpha A antibody|Heat shock protein beta 4 antibody|Heat shock protein beta-4 antibody|HSPB 4 antibody|HspB4 antibody|short form antibody|Zonular Central Nuclear Cataract antibody|Anti-alpha A Crystallin antibody (ab5595)

SCBT cat No: sc-28306|sc-22389|sc-22743|sc-22388|sc-398304|sc-69687|

Name

Rabbit polyclonal CRYAA antibody

Catalogue No.

A5725

Reactivity

Human, Mouse, Rat, Zebrafish

Immunogen

Recombinant protein of human CRYAA

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 20kDa/Observed: Refer to figures

Formulation

CRYAA antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

CRYAA antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CRYA1 antibody, HSPB4 antibody, CTRCT9 antibody

Database links

Human UniProt/Swiss-Prot:P02489

Protein names

CRYA1, HSPB4, CTRCT9

Protein function

Contributes to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions.

Protein tissue specificity

Expressed in eye lens.

Protein sequence and domain

Belongs to the small heat shock protein (HSP20) family.

Protein post-translational modifications

O-glycosylated; contains N-acetylglucosamine side chains.; Deamidation of Asn-101 in lens occurs mostly during the first 30 years of age, followed by a small additional amount of deamidation (approximately 5%) during the next approximately 38 years, resulting in a maximum of approximately 50% deamidation during the lifetime of the individual.; Phosphorylation on Ser-122 seems to be developmentally regulated. Absent in the first months of life, it appears during the first 12 years of human lifetime. The relative amount of phosphorylated form versus unphosphorylated form does not change over the lifetime of the individual.; Acetylation at Lys-70 seems to increase chaperone activity.; Undergoes age-dependent proteolytical cleavage at the C-terminus. Alpha-crystallin A(1-172) is the most predominant form produced most rapidly during the first 12 years of age and after this age is present in approximatley 50% of the lens molecules.

Protein cellular localization

Cytoplasm . Nucleus . Note: Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.

Background

Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC).

Note

AntibodyPlus can customize CRYAA antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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