Rabbit polyclonal CTCF antibody (A1133)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IP,ChIP
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal CTCF antibody (A1133)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IP,ChIP

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IP 1:20 – 1:50 ChIP 1:20 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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CTCF antibody
11 zinc finger protein antibody|11 zinc finger transcriptional repressor antibody|11-zinc finger protein antibody|CCCTC binding factor (zinc finger protein) antibody|CCCTC binding factor antibody|CCCTC-binding factor antibody|Ctcf antibody|CTCF_HUMAN antibody|CTCFL paralog antibody|MRD21 antibody|Transcriptional repressor CTCF antibody|Anti-CTCF antibody – ChIP Grade (ab70303)

SCBT cat No: sc-136284|sc-271514|sc-15914|sc-398149|sc-15913|sc-271474|sc-28198|sc-5916|

Name

Rabbit polyclonal CTCF antibody

Catalogue No.

A1133

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human CTCF

Host

Rabbit

Applications

WB, IHC, IP, ChIP

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IP 1:20 – 1:50
ChIP 1:20 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 83kDa/Observed: Refer to Figures

Formulation

CTCF antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

CTCF antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CTCF antibody

Database links

Human UniProt/Swiss-Prot:P49711

Protein names

CTCF

Protein function

Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19.

Protein tissue specificity

Ubiquitous. Absent in primary spermatocytes.

Protein sequence and domain

The 11 zinc fingers are highly conserved among vertebrates, exhibiting almost identical amino acid sequences. Different subsets or combination of individual zinc fingers gives the ability to CTCF to recognize multiple DNA target sites.Belongs to the CTCF zinc-finger protein family.; Contains 11 C2H2-type zinc fingers.

Protein post-translational modifications

Sumoylated on Lys-74 and Lys-689; sumoylation of CTCF contributes to the repressive function of CTCF on the MYC P2 promoter.

Protein cellular localization

Nucleus, nucleoplasm. Chromosome. Chromosome, centromere. Note: May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase.

Background

CCCTC-binding factor (CTCF) and its paralog, the Brother of the Regulator of Imprinted Sites (BORIS), are highly conserved transcription factors that regulate transcriptional activation and repression, insulator function, and imprinting control regions (ICRs) (1-4). Although they have divergent amino and carboxy termini, both proteins contain 11 conserved zinc finger domains that work in combination to bind the same DNA elements (1). CTCF is ubiquitously expressed and contributes to transcriptional regulation of cell-growth regulated genes, including c-myc, p19/ARF, p16/INK4A, BRCA1, p53, p27, E2F1, and TERT (1). CTCF also binds to and is required for the enhancer-blocking activity of all known insulator elements and ICRs, including the H19/IgF2, Prader-Willi/Angelman syndrome, and Inactive X-Specific Transcript (XIST) anti-sense loci (5-7). CTCF DNA-binding is sensitive to DNA methylation, a mark that determines selection of the imprinted allele (maternal vs. paternal) (1). The various functions of CTCF are regulated by at least two different post-translational modifications. Poly(ADP-ribosyl)ation of CTCF is required for insulator function (8). Phosphorylation of Ser612 by protein kinase CK2 facilitates a switch of CTCF from a transcriptional repressor to an activator at the c-myc promoter (9).

Research area

All research areas>Transcription Regulators>CTCF
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize CTCF antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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