Rabbit polyclonal CXCR4 antibody (A1303)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal CXCR4 antibody (A1303)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:1000 IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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CD184 antibody, D2S201E antibody, FB22 antibody, HM89 antibody, HSY3RR antibody, LAP3 antibody, LCR1 antibody, LESTR antibody, NPY3R antibody, NPYR antibody, NPYRL antibody, NPYY3R antibody, WHIM antibody
C-X-C chemokine receptor type 4 antibody|CD184 antibody|CD184 antigen antibody|Chemokine (C X C motif) receptor 4 antibody|Chemokine CXC Motif Receptor 4 antibody|CXC-R4 antibody|CXCR-4 antibody|CXCR4 antibody|CXCR4_HUMAN antibody|D2S201E antibody|FB22 antibody|Fusin antibody|HM89 antibody|HSY3RR antibody|LAP 3 antibody|LAP3 antibody|LCR1 antibody|LESTR antibody|Leukocyte derived seven transmembrane domain receptor antibody|Leukocyte-derived seven transmembrane domain receptor antibody|Lipopolysaccharide associated protein 3 antibody|Neuropeptide Y receptor Y3 antibody|NPY3R antibody|NPYR antibody|NPYRL antibody|NPYY3 antibody|NPYY3R antibody|Probable G protein coupled receptor lcr1 homolog antibody|SDF 1 receptor antibody|SDF-1 receptor antibody|SEVEN-TRANSMEMBRANE-SEGMENT RECEPTOR antibody|Stromal cell derived factor 1 receptor antibody|Stromal cell-derived factor 1 receptor antibody|WHIM antibody|WHIMS antibody|Anti-CXCR4 antibody [UMB2] (ab124824)

SCBT cat No: sc-12764|sc-53534|sc-6191|sc-6190|sc-6279|sc-9046|

Name

Rabbit polyclonal CXCR4 antibody

Catalogue No.

A1303

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human CXCR4

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:1000
IHC 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 40kDa/Observed: Refer to Figures

Formulation

CXCR4 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

CXCR4 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CD184 antibody, D2S201E antibody, FB22 antibody, HM89 antibody, HSY3RR antibody, LAP3 antibody, LCR1 antibody, LESTR antibody, NPY3R antibody, NPYR antibody, NPYRL antibody, NPYY3R antibody, WHIM antibody

Database links

Human UniProt/Swiss-Prot:P61073

Protein names

CD184, D2S201E, FB22, HM89, HSY3RR, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM

Protein function

Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival.; (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) for human immunodeficiency virus-1/HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus . Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes .

Protein tissue specificity

Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.

Protein sequence and domain

The amino-terminus is critical for ligand binding. Residues in all four extracellular regions contribute to HIV-1 coreceptor activity.Belongs to the G-protein coupled receptor 1 family.

Protein post-translational modifications

Phosphorylated on agonist stimulation. Rapidly phosphorylated on serine and threonine residues in the C-terminal. Phosphorylation at Ser-324 and Ser-325 leads to recruitment of ITCH, ubiquitination and protein degradation.; Ubiquitinated by ITCH at the cell membrane on agonist stimulation. The ubiquitin-dependent mechanism, endosomal sorting complex required for transport (ESCRT), then targets CXCR4 for lysosomal degradation. This process is dependent also on prior Ser-/Thr-phosphorylation in the C-terminal of CXCR4. Also binding of ARRB1 to STAM negatively regulates CXCR4 sorting to lysosomes though modulating ubiquitination of SFR5S.; Sulfation on Tyr-21 is required for efficient binding of CXCL12/SDF-1alpha and promotes its dimerization. Tyr-7 and Tyr-12 are sulfated in a sequential manner after Tyr-21 is almost fully sulfated, with the binding affinity for CXCL12/SDF-1alpha increasing with the number of sulfotyrosines present. Sulfotyrosines Tyr-7 and Tyr-12 occupy clefts on opposing CXCL12 subunits, thus bridging the CXCL12 dimer interface and promoting CXCL12 dimerization.; O- and N-glycosylated. Asn-11 is the principal site of N-glycosylation. There appears to be very little or no glycosylation on Asn-176. N-glycosylation masks coreceptor function in both X4 and R5 laboratory-adapted and primary HIV-1 strains through inhibiting interaction with their Env glycoproteins. The O-glycosylation chondroitin sulfate attachment does not affect interaction with CXCL12/SDF-1alpha nor its coreceptor activity.

Protein cellular localization

Cell membrane; Multi-pass membrane protein. Cell junction. Early endosome. Late endosome. Lysosome. Note: In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated. In the presence of antigen, distributes to the immunological synapse forming at the T-cell-APC contact area, where it localizes at the peripheral and distal supramolecular activation cluster (SMAC).

Background

This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Research area

All research areas>Membrane Receptors>CXCR
(View all antibody categories related to Membrane Receptors)

Note

AntibodyPlus can customize CXCR4 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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