Rabbit Polyclonal Cyclin E1 antibody (STJ92541)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Cyclin E1 antibody (STJ92541)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Cyclin E1 Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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CCNE1 antibody, CCNE antibody,|CCNE antibody|Ccne1 antibody|CCNE1_HUMAN antibody|cyclin E variant ex5del antibody|cyclin E variant ex7del antibody|Cyclin E1 antibody|Cyclin Es antibody|Cyclin Et antibody|CyclinE antibody|G1/S specific cyclin E antibody|G1/S-specific cyclin-E1 antibody|Anti-Cyclin E1 antibody [HE12] (ab3927)
SCBT cat No: sc-48420|sc-198|sc-377100|sc-20684|sc-248|sc-247|sc-481|sc-28351|

Name

Cyclin E1 Polyclonal Antibody

Catalogue No.

STJ92541

Reactivity

Human, Mouse, Rat

Specificity

Cyclin E1 Polyclonal Antibody detects endogenous levels of Cyclin E1 protein.

Immunogen

Synthesized peptide derived from Cyclin E1 at AA range 60-140

Host

Rabbit

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

47 kDa

Formulation

Cyclin E1 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Cyclin E1 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

G1/S-specific cyclin-E1 antibody

Database links

Human UniProt/Swiss-Prot:P24864;Mouse UniPort/Swiss-Prot: Q61457;Rat UniProt/Swiss-Port: B1WC54;Human Entrez Gene: 898;Mouse Entrez Gene: 12447;Rat Entrez Gene: Rn.15455

Protein names

G1/S-specific cyclin-E1

Protein function

Essential for the control of the cell cycle at the G1/S (start) transition.

Protein tissue specificity

Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.

Protein sequence and domain

Belongs to the cyclin family. Cyclin E subfamily.

Protein post-translational modifications

Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7. / Ubiquitinated by UHRF2; appears to occur independently of phosphorylation.

Protein cellular localization

Nucleus

Research area

All research areas>Cell Cycle Proteins>Cyclin
(View all antibody categories related to Cell Cycle Proteins)

Note

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Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Rat lung tissue

1: Cyclin E1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat spleen tissue

1: Cyclin E1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.


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