Rabbit Polyclonal Cytokeratin 18 antibody (STJ92631)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Cytokeratin 18 antibody (STJ92631)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IP, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IP 1:200-500; IF 1:200-1:1000; ELISA 1:40000;

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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KRT18 antibody, CYK18 antibody, PIG46 antibody,|Cell proliferation inducing gene 46 protein antibody|Cell proliferation inducing protein 46 antibody|Cell proliferation-inducing gene 46 protein antibody|CK 18 antibody|CK-18 antibody|CK18 antibody|CYK 18 antibody|CYK18 antibody|Cytokeratin 18 antibody|Cytokeratin endo B antibody|Cytokeratin-18 antibody|K 18 antibody|K18 antibody|K1C18_HUMAN antibody|KA18 antibody|Keratin 18 antibody|Keratin 18, type I antibody|Keratin D antibody|keratin, type I cytoskeletal 18 antibody|Keratin-18 antibody|Krt18 antibody|Anti-Cytokeratin 18 antibody [C-04] (ab668)
SCBT cat No: sc-53254|sc-377224|sc-271937|sc-53255|sc-70915|sc-393091|sc-58726|sc-393002|sc-366511|sc-101461|sc-101931|sc-70917|sc-58727|sc-398871|sc-51582|sc-51583|sc-6259|sc-28264|sc-53256|sc-31700|sc-31701|sc-32722|

Name

Cytokeratin 18 Polyclonal Antibody

Catalogue No.

STJ92631

Reactivity

Human, Mouse, Rat

Specificity

Cytokeratin 18 Polyclonal Antibody detects endogenous levels of Cytokeratin 18 protein.

Immunogen

Synthesized peptide derived from Cytokeratin 18 at AA range 10-90

Host

Rabbit

Applications

WB, IHC, IP, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IP 1:200-500; IF 1:200-1:1000; ELISA 1:40000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

47 kDa

Formulation

Cytokeratin 18 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Cytokeratin 18 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Keratin, type I cytoskeletal 18 antibody, Cell proliferation-inducing gene 46 protein antibody, Cytokeratin-18 antibody, CK-18 antibody, Keratin-18 antibody, K18 antibody

Database links

Human UniProt/Swiss-Prot:P05783;Mouse UniPort/Swiss-Prot: P05784;Rat UniProt/Swiss-Port: Q5BJY9;Human Entrez Gene: 3875;Mouse Entrez Gene: 16668;Rat Entrez Gene: Rn.103924

Protein names

Keratin, type I cytoskeletal 18 , Cell proliferation-inducing gene 46 protein , Cytokeratin-18 , CK-18 , Keratin-18 , K18

Protein function

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Protein tissue specificity

Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

Involvement in disease

Cirrhosis (CIRRH) [MIM:215600]: A liver disease characterized by severe panlobular liver-cell swelling with Mallory body formation, prominent pericellular fibrosis, and marked deposits of copper. Clinical features include abdomen swelling, jaundice and pulmonary hypertension. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the intermediate filament family.

Protein post-translational modifications

Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. / Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. / O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.

Protein cellular localization

Cytoplasm > perinuclear region / Nucleus > nucleolus

Research area

All research areas>Structural Proteins>Cytokeratin
(View all antibody categories related to Structural Proteins)

Note

AntibodyPlus can customize Cytokeratin 18 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Rat lung tissue

1: Cytokeratin 18 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat lung tissue

1: Cytokeratin 18 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat kidney tissue

1: Cytokeratin 18 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat kidney tissue

1: Cytokeratin 18 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse kidney tissue

1: Cytokeratin 18 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse kidney tissue

1: Cytokeratin 18 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human Tonsil tissue

1: Cytokeratin 18 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: Cytokeratin 18 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat brain tissue

1: Cytokeratin 18 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse colon tissue

1: Cytokeratin 18 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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