Rabbit polyclonal DDB1 antibody (A2896)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal DDB1 antibody (A2896)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:1000 IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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XPE antibody,DDBA antibody,XAP1 antibody,XPCE antibody,XPE-BF antibody,UV-DDB1 antibody
Damage specific DNA binding protein 1 antibody|Damage-specific DNA-binding protein 1 antibody|DDB 1 antibody|DDB p127 subunit antibody|Ddb1 antibody|DDB1_HUMAN antibody|DDBa antibody|DNA damage binding protein 1 antibody|DNA damage-binding protein 1 antibody|DNA damage-binding protein a antibody|HBV X-associated protein 1 antibody|UV damaged DNA binding factor antibody|UV damaged DNA binding protein 1 antibody|UV DDB 1 antibody|UV DDB1 antibody|UV-damaged DNA-binding factor antibody|UV-damaged DNA-binding protein 1 antibody|UV-DDB 1 antibody|X associated protein 1 antibody|XAP 1 antibody|XAP-1 antibody|XAP1 antibody|Xeroderma pigmentosum group E complementing protein antibody|Xeroderma pigmentosum group E-complementing protein antibody|XPCe antibody|XPE antibody|XPE BF antibody|XPE binding factor antibody|XPE-BF antibody|XPE-binding factor antibody|Anti-DDB1 antibody [EPR6089] (ab109027)

SCBT cat No: sc-136180|sc-137142|sc-137132|sc-376860|sc-25367|sc-16292|

Name

Rabbit polyclonal DDB1 antibody

Catalogue No.

A2896

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human DDB1

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:1000
IHC 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 127kDa/Observed: Refer to Figures

Formulation

DDB1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

DDB1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

XPE antibody,DDBA antibody,XAP1 antibody,XPCE antibody,XPE-BF antibody,UV-DDB1 antibody

Database links

Human UniProt/Swiss-Prot:Q16531

Protein names

XPE,DDBA,XAP1,XPCE,XPE-BF,UV-DDB1

Protein function

Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.

Protein sequence and domain

The core of the protein consists of three WD40 beta-propeller domains.Belongs to the DDB1 family.

Protein post-translational modifications

Phosphorylated by ABL1.; Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis.

Protein cellular localization

Cytoplasm. Nucleus. Note: Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.

Background

The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) – an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins.

Note

AntibodyPlus can customize DDB1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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