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Rabbit polyclonal DDX1 antibody (A6575)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC,IF
WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:10 – 1:100
Recommended protocols: check protocols
Click or hover above images to see image description for Rabbit polyclonal DDX1 antibody.
Check alternative names for the antibodyExpand
DBP-RB antibody, UKVH5d antibody
ATP dependent helicase DDX1 antibody|ATP-dependent RNA helicase DDX1 antibody|DBP RB antibody|DBP-RB antibody|DDX1 antibody|DDX1_HUMAN antibody|DEAD (Asp Glu Ala Asp) box polypeptide 1 antibody|DEAD box 1 antibody|DEAD box polypeptide 1 antibody|DEAD box protein 1 antibody|DEAD box protein retinoblastoma antibody|DEAD/H (Asp Glu Ala Asp/His) box polypeptide 1 antibody|Anti-DDX1 antibody (ab31963)
SCBT cat No: sc-135878|sc-271438|sc-271393|sc-134752|sc-49817|
Rabbit polyclonal DDX1 antibody
|Catalogue No.|| |
Human, Mouse, Rat
Recombinant protein of human DDX1
WB, IHC, IF
|Recommended dilution|| |
WB 1:500 – 1:2000
|Molecular weight|| |
Predicted: 82kDa/Observed: Refer to figures
DDX1 antibody was tube-contained.
DDX1 antibody was purified using affinity purification.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
DBP-RB antibody, UKVH5d antibody
|Database links|| |
|Protein names|| |
|Protein function|| |
Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5′ single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3′-end cleavage and polyadenylation of pre-mRNAs. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB: together with archease (ZBTB8OS), acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation . Required for HIV-1 Rev function as well as for HIV-1 replication. Binds to the RRE sequence of HIV-1 mRNAs.
|Protein tissue specificity|| |
Highest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord.
|Protein sequence and domain|| |
The helicase domain is involved in the stimulation of RELA transcriptional activity.Belongs to the DEAD box helicase family. DDX1 subfamily.; Contains 1 B30.2/SPRY domain.; Contains 1 helicase ATP-binding domain.; Contains 1 helicase C-terminal domain.
|Protein post-translational modifications|| |
Phosphorylated. Phosphorylated by ATM kinase; phosphorylation is increased in response to ionizing radiation (IR).
|Protein cellular localization|| |
Nucleus. Cytoplasm. Cytoplasmic granule. Note: Localized with MBNL1, TIAL1 and YBX1 in stress granules upon stress. Localized with CSTF2 in cleavage bodies. Forms large aggregates called DDX1 bodies. Relocalized into multiple foci (IR-induced foci or IRIF) after IR treatment, a process that depends on the presence of chromosomal DNA and/or RNA-DNA duplexes. Relocalized at sites of DNA double-strand breaks (DSBs) in an ATM-dependent manner after IR treatment. Colocalized with RELA in the nucleus upon TNF-alpha induction. Relocalized to the cytoplasm with a perinuclear staining pattern in avian infectious bronchitis virus (IBV)-infected cells. Required for proper localization of HIV-1 Rev. Enters into the nucleus in case of active transcription while it accumulates in cytosol when transcription level is low .
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.
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|Product type|| |
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