Rabbit polyclonal DDX1 antibody (A6575)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal DDX1 antibody (A6575)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:10 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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DBP-RB antibody, UKVH5d antibody
ATP dependent helicase DDX1 antibody|ATP-dependent RNA helicase DDX1 antibody|DBP RB antibody|DBP-RB antibody|DDX1 antibody|DDX1_HUMAN antibody|DEAD (Asp Glu Ala Asp) box polypeptide 1 antibody|DEAD box 1 antibody|DEAD box polypeptide 1 antibody|DEAD box protein 1 antibody|DEAD box protein retinoblastoma antibody|DEAD/H (Asp Glu Ala Asp/His) box polypeptide 1 antibody|Anti-DDX1 antibody (ab31963)

SCBT cat No: sc-135878|sc-271438|sc-271393|sc-134752|sc-49817|

Name

Rabbit polyclonal DDX1 antibody

Catalogue No.

A6575

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human DDX1

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:10 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 82kDa/Observed: Refer to figures

Formulation

DDX1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

DDX1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

DBP-RB antibody, UKVH5d antibody

Database links

Human UniProt/Swiss-Prot:Q92499

Protein names

DBP-RB, UKVH5d

Protein function

Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5′ single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3′-end cleavage and polyadenylation of pre-mRNAs. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB: together with archease (ZBTB8OS), acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation . Required for HIV-1 Rev function as well as for HIV-1 replication. Binds to the RRE sequence of HIV-1 mRNAs.

Protein tissue specificity

Highest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord.

Protein sequence and domain

The helicase domain is involved in the stimulation of RELA transcriptional activity.Belongs to the DEAD box helicase family. DDX1 subfamily.; Contains 1 B30.2/SPRY domain.; Contains 1 helicase ATP-binding domain.; Contains 1 helicase C-terminal domain.

Protein post-translational modifications

Phosphorylated. Phosphorylated by ATM kinase; phosphorylation is increased in response to ionizing radiation (IR).

Protein cellular localization

Nucleus. Cytoplasm. Cytoplasmic granule. Note: Localized with MBNL1, TIAL1 and YBX1 in stress granules upon stress. Localized with CSTF2 in cleavage bodies. Forms large aggregates called DDX1 bodies. Relocalized into multiple foci (IR-induced foci or IRIF) after IR treatment, a process that depends on the presence of chromosomal DNA and/or RNA-DNA duplexes. Relocalized at sites of DNA double-strand breaks (DSBs) in an ATM-dependent manner after IR treatment. Colocalized with RELA in the nucleus upon TNF-alpha induction. Relocalized to the cytoplasm with a perinuclear staining pattern in avian infectious bronchitis virus (IBV)-infected cells. Required for proper localization of HIV-1 Rev. Enters into the nucleus in case of active transcription while it accumulates in cytosol when transcription level is low .

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.

Research area

All research areas>Transcription Regulators>DDX
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize DDX1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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