Rabbit polyclonal DIAPH1 antibody (A5772)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal DIAPH1 antibody (A5772)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal DIAPH1 antibody.

Alternative names:

Check alternative names for the antibody

Expand

DIA1 antibody, DRF1 antibody, DFNA1 antibody, LFHL1 antibody, hDIA1 antibody
DIAPH1 antibody|DRF1 antibody|FLJ25265 antibody|hDIA1 antibody|LFHL1 antibody|low frequency hearing loss 1 antibody|p140DIA antibody|Protein diaphanous homolog 1 antibody|Anti-DIAPH1 antibody (ab11173)

SCBT cat No: sc-10885|sc-373895|sc-373807|sc-28775|sc-10888|sc-10886|

Name

Rabbit polyclonal DIAPH1 antibody

Catalogue No.

A5772

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human DIAPH1

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 141kDa/Observed: Refer to Figures

Formulation

DIAPH1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

DIAPH1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

DIA1 antibody, DRF1 antibody, DFNA1 antibody, LFHL1 antibody, hDIA1 antibody

Database links

Human UniProt/Swiss-Prot:O60610

Protein names

DIA1, DRF1, DFNA1, LFHL1, hDIA1

Protein function

Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape . Plays a role in brain development .

Protein tissue specificity

Expressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea.

Protein sequence and domain

The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).Belongs to the formin homology family. Diaphanous subfamily.; Contains 1 DAD (diaphanous autoregulatory) domain.; Contains 1 FH1 (formin homology 1) domain.; Contains 1 FH2 (formin homology 2) domain.; Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.

Protein post-translational modifications

Phosphorylation at Thr-768 is stimulated by cAMP and regulates stability, complex formation and mitochondrial movement.

Protein cellular localization

Cell membrane . Cell projection, ruffle membrane . Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Note: Membrane ruffles, especially at the tip of ruffles, of motile cells.

Background

This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Note

AntibodyPlus can customize DIAPH1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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