Rabbit polyclonal DLC1 antibody (A1921)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal DLC1 antibody (A1921)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal DLC1 antibody.

Alternative names:

Check alternative names for the antibody

Expand

HP antibody, ARHGAP7 antibody, STARD12 antibody, p122-RhoGAP antibody
ARHGAP 7 antibody|STARD 12 antibody|StARD12 antibody|START domain containing protein 12 antibody|START domain-containing protein 12 antibody|Anti-DLC1 antibody (ab21200)

SCBT cat No: sc-271915|sc-28434|sc-32931|

Name

Rabbit polyclonal DLC1 antibody

Catalogue No.

A1921

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human DLC1

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 171kDa/Observed: Refer to Figures

Formulation

DLC1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

DLC1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

HP antibody, ARHGAP7 antibody, STARD12 antibody, p122-RhoGAP antibody

Database links

Human UniProt/Swiss-Prot:Q96QB1

Protein names

HP, ARHGAP7, STARD12, p122-RhoGAP

Protein function

Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality.

Protein tissue specificity

Highest level of expression in the spleen, with rather lower levels in prostate, testis, ovary, small intestine and colon, but none in the thymus.

Protein sequence and domain

The SAM domain mediates interaction with EF1A1, and functions as an autoinhibitory regulator of RhoGAP Activity.; The polybasic cluster is required for activation and mediates binding to phosphatidylinositol-4,5-bisphosphate (PI(4,5)P(2)) containing membranes.Contains 1 Rho-GAP domain.; Contains 1 SAM (sterile alpha motif) domain.; Contains 1 START domain.

Protein cellular localization

Cytoplasm. Cell junction, focal adhesion. Membrane; Peripheral membrane protein. Note: Colocalizes with EF1A1 at actin-rich regions in the cell periphery.

Background

This gene encodes a GTPase-activating protein (GAP) that is a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins. GAP family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. This gene functions as a tumor suppressor gene in a number of common cancers, including prostate, lung, colorectal, and breast cancers. Multiple transcript variants due to alternative promoters and alternative splicing have been found for this gene.

Research area

All research areas>GDP/GTP Binding Proteins>DLC
(View all antibody categories related to GDP/GTP Binding Proteins)

Note

AntibodyPlus can customize DLC1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

Reviews


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Antibody customer reviews & validation data - 41

Validation data ID: 41

Submission date: 2016-12-23

View Details

Result figure:
DLC1.pdf

Figure description:

By immunoblotting three bands of around 37, 80, and 100 kDa were detected by A1921 ABclonal DLC1 polyclonal antibody in lysates of epithelial cells.

Product name

DLC1 Polyclonal Antibody

Product catalog No.

A1921

Product type

Primary antibody

Supplier

ABclonal

Application type

Western Blot

Antibody specificity rating

Poor

Antibody overall rating

Poor

Your comments

Unfortunately, by western blotting the anti-DLC1 antibody does not react with the proper antigen in lysates of mouse embryonic epithelial cells. These cells do express DLC1 (confirmed by RNA-seq).

Detailed protocol

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