Rabbit polyclonal DLG1 antibody (A8542)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal DLG1 antibody (A8542)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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SCBT cat No: sc-9961|sc-514478|sc-25661|sc-26530|sc-26532|

Name

Rabbit polyclonal DLG1 antibody

Catalogue No.

A8542

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human DLG1

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 100kDa/Observed: Refer to figures

Formulation

DLG1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

DLG1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Database links

Human UniProt/Swiss-Prot:Q12959

Protein function

Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel.

Protein tissue specificity

Abundantly expressed in atrial myocardium (at protein level). Expressed in lung fibroblasts, cervical epithelial and B-cells (at protein level). Widely expressed, with isoforms displaying different expression profiles.

Protein sequence and domain

The alternatively spliced domain I3 corresponding to amino acids (636-669) of isoform 4 is an EPB41 binding site mediating association to membranes in polarized and non-polarized cells.; The PDZ domains may also mediate association to membranes by binding to EPB41 and ADGRA2 together with the L27 domain that binds CASK and DLG2.; The L27 domain may regulate DLG1 self-association. The N-terminal alternatively spliced region is capable of binding several SH3 domains and also moderates the level of protein oligomerization.Belongs to the MAGUK family.; Contains 1 guanylate kinase-like domain.; Contains 1 L27 domain.; Contains 3 PDZ (DHR) domains.; Contains 1 SH3 domain.

Protein post-translational modifications

Phosphorylated by MAPK12. Phosphorylation of Ser-232 regulates association with GRIN2A (By similarity).

Protein cellular localization

Membrane ; Peripheral membrane protein . Basolateral cell membrane . Endoplasmic reticulum membrane . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Cell junction, synapse. Cell membrane, sarcolemma. Note: Colocalizes with EPB41 at regions of intercellular contacts. Basolateral in epithelial cells. May also associate with endoplasmic reticulum membranes. Mainly found in neurons soma, moderately found at postsynaptic densities (By similarity).

Note

AntibodyPlus can customize DLG1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

Reviews


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Antibody customer reviews & validation data - 138

Validation data ID: 138

Submission date: 2017-03-03

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Result figure:
Result figure

Figure description:

IP studies were done to test if endogenous DLG1 is co precipitated with the protein of our interest. As evident from our blot DLG1 is enriched with the protein of our interest ( lane4) and not in the negative control ( lane3).

Product name

Rabbit polyclonal DLG1 antibody

Product catalog No.

A8542

Product type

Primary antibody

Supplier

ABclonal

Application type

Western Blot

Antibody specificity rating

Good

Antibody overall rating

Good

Your comments

This antibody was used to detect DLG1 from a lysates prepared from two cell lines HEK 293T and MDA MB231, that were mixed and used for immunoprecipitation studies

Detailed protocol

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