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Rabbit polyclonal DNMT3A antibody (A2065)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC,IF
WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:20 – 1:100
Recommended protocols: check protocols
Click or hover above images to see image description for Rabbit polyclonal DNMT3A antibody.
Check alternative names for the antibodyExpand
DNMT3A2 antibody, M.HsaIIIA antibody
DNA (cytosine 5) methyltransferase 3 alpha antibody|DNA (cytosine 5) methyltransferase 3A antibody|DNA (cytosine-5)-methyltransferase 3A antibody|DNA cytosine methyltransferase 3A2 antibody|DNA methyltransferase 3 alpha antibody|DNA methyltransferase 3a antibody|DNA methyltransferase HsaIIIA antibody|DNA MTase HsaIIIA antibody|DNM3A_HUMAN antibody|DNMT 3a antibody|DNMT antibody|Dnmt3a antibody|DNMT3A2 antibody|M.HsaIIIA antibody|MCMT antibody|OTTHUMP00000201149 antibody|TBRS antibody|Anti-Dnmt3a antibody [64B1446] – ChIP Grade (ab13888)
SCBT cat No: sc-52921|sc-56656|sc-373905|sc-365769|sc-10232|sc-20703|sc-10231|sc-10234|
Rabbit polyclonal DNMT3A antibody
|Catalogue No.|| |
Recombinant protein of human DNMT3A
WB, IHC, IF
|Recommended dilution|| |
WB 1:500 – 1:2000
|Molecular weight|| |
Predicted: 102kDa/Observed: Refer to Figures
DNMT3A antibody was tube-contained.
DNMT3A antibody was purified using affinity purification.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
DNMT3A2 antibody, M.HsaIIIA antibody
|Database links|| |
|Protein names|| |
|Protein function|| |
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated ‘Lys-36’ of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.
|Protein tissue specificity|| |
Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
|Protein sequence and domain|| |
The PWWP domain is essential for targeting to pericentric heterochromatin. It specifically recognizes and binds trimethylated ‘Lys-36’ of histone H3 (H3K36me3) (By similarity).Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.; Contains 1 ADD domain.; Contains 1 GATA-type zinc finger.; Contains 1 PHD-type zinc finger.; Contains 1 PWWP domain.; Contains 1 SAM-dependent MTase C5-type domain.
|Protein post-translational modifications|| |
Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
|Protein cellular localization|| |
Nucleus . Cytoplasm . Note: Accumulates in the major satellite repeats at pericentric heterochromatin.
Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting and development (1,2). Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2 and DNMT3 (1,2). DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of DNA. DNMT2 is expressed at low levels in adult somatic tissues and its inactivation affects neither de novo nor maintenance DNA methylation. DNMT1, DNMT3A and DNMT3B together form a protein complex that interacts with histone deacetylases (HDAC1, HDAC2, Sin3A), transcriptional repressor proteins (RB, TAZ-1) and heterochromatin proteins (HP1, SUV39H1), to maintain proper levels of DNA methylation and facilitate gene silencing (3-8). Improper DNA methylation contributes to diseased states such as cancer (1,2). Hypermethylation of promoter CpG islands within tumor suppressor genes correlates with gene silencing and the development of cancer. In addition, hypomethylation of bulk genomic DNA correlates with and may contribute to the onset of cancer. DNMT1, DNMT3A and DNMT3B are over-expressed in many cancers, including acute and chronic myelogenous leukemias, in addition to colon, breast and stomach carcinomas (9-12).
AntibodyPlus can customize DNMT3A antibody according to your requirement, including bulk product size,etc. Please contact email@example.com. AntibodyPlus provides antibody trial sample for your own antibody validation.
|Product type|| |
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