Rabbit polyclonal DNMT3B antibody (A2899)

$69.00$259.00

Reactivity: Human,Mouse
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal DNMT3B antibody (A2899)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:200 – 1:1000 IHC 1:20 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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ICF antibody, ICF1 antibody, M.HsaIIIB antibody
Cytosine 5methyltransferase 3B antibody|DNA antibody|DNA (cytosine 5) methyltransferase 3 beta antibody|DNA (cytosine 5)-methyltransferase 3B antibody|DNA (cytosine-5)-methyltransferase 3B antibody|DNA methyltransferase HsaIIIB antibody|DNA MTase HsaIIIB antibody|DNM3B_HUMAN antibody|Dnmt3b antibody|EC 2.1.1.37 antibody|ICF antibody|ICF1 antibody|M.HsaIIIB antibody|MGC124407 antibody|RP23-89H14.3 antibody|Anti-Dnmt3b antibody – ChIP Grade (ab2851)

SCBT cat No: sc-81252|sc-70984|sc-52922|sc-393279|sc-393845|sc-376043|sc-20704|sc-10235|sc-130740|sc-10236|

Name

Rabbit polyclonal DNMT3B antibody

Catalogue No.

A2899

Reactivity

Human, Mouse

Immunogen

A synthetic peptide of human DNMT3B

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:200 – 1:1000
IHC 1:20 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 96kDa/Observed: Refer to Figures

Formulation

DNMT3B antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

DNMT3B antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

ICF antibody, ICF1 antibody, M.HsaIIIB antibody

Database links

Human UniProt/Swiss-Prot:Q9UBC3

Protein names

ICF, ICF1, M.HsaIIIB

Protein function

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1.

Protein tissue specificity

Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.

Protein sequence and domain

The PWWP domain is essential for targeting to pericentric heterochromatin.Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.; Contains 1 ADD domain.; Contains 1 GATA-type zinc finger.; Contains 1 PHD-type zinc finger.; Contains 1 PWWP domain.; Contains 1 SAM-dependent MTase C5-type domain.

Protein post-translational modifications

Sumoylated.; Citrullinated by PADI4.

Protein cellular localization

Nucleus .

Background

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq, May 2011]

Research area

All research areas>Transcription Regulators>Dnmt
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize DNMT3B antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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