Rabbit Polyclonal E2F-1 antibody (STJ98642)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal E2F-1 antibody (STJ98642)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:5000-10000; ELISA 1:10000

Recommended protocols: check protocols

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Alternative names:

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E2F1 antibody, RBBP3 antibody,|DmelCG6376 antibody|Dmel_CG6376 antibody|drosE2F1 antibody|E(Sev-CycE)3A antibody|E(var)3-93E antibody|E2-promoter binding facto antibody|E2F 1 antibody|E2F transcription factor 1 antibody|E2F-1 antibody|E2f-PA antibody|E2f-PB antibody|E2f-PC antibody|E2F1 antibody|E2f1 E2F transcription factor 1 antibody|E2F1_HUMAN antibody|Evar(3)164 antibody|KIAA4009 antibody|l(3)07172 antibody|l(3)j3B1 antibody|l(3)j3C2 antibody|l(3)rM729 antibody|mKIAA4009 antibody|OTTHUMP00000030661 antibody|PBR3 antibody|PRB binding protein E2F 1 antibody|PRB-binding protein E2F-1 antibody|RBAP 1 antibody|RBAP-1 antibody|RBAP1 antibody|RBBP-3 antibody|RBBP3 antibody|RBP 3 antibody|RBP3 antibody|Retinoblastoma-associated protein 1 antibody|Retinoblastoma-binding protein 3 antibody|Transcription factor E2F1 antibody|Anti-E2F1 antibody [EPR3818(3)] (ab179445)
SCBT cat No: sc-193|sc-137059|sc-137110|sc-137060|sc-22820|sc-56661|sc-56662|sc-251|sc-515402|

Name

E2F-1 Polyclonal Antibody

Catalogue No.

STJ98642

Reactivity

Human, Mouse, Rat

Specificity

The antibody detects endogenous E2F-1 protein

Immunogen

Synthetic peptide from AA range 100-170

Host

Rabbit

Applications

WB, ELISA

Recommended dilution

WB 1:5000-10000; ELISA 1:10000

Clonality

Polyclonal

Conjugation

Unconjugated

Formulation

E2F-1 Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Concentration

1 mg/ml

Purification

E2F-1 Antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Transcription factor E2F1 antibody, E2F-1 antibody, PBR3 antibody, Retinoblastoma-associated protein 1 antibody, RBAP-1 antibody, Retinoblastoma-binding protein 3 antibody, RBBP-3 antibody, pRB-binding protein E2F-1 antibody

Database links

Human UniProt/Swiss-Prot:Q01094;Mouse UniPort/Swiss-Prot: Q61501;Rat UniProt/Swiss-Port: O09139;Human Entrez Gene: 1869;Mouse Entrez Gene: 13555;Rat Entrez Gene: Rn.72471

Protein names

Transcription factor E2F1 , E2F-1 , PBR3 , Retinoblastoma-associated protein 1 , RBAP-1 , Retinoblastoma-binding protein 3 , RBBP-3 , pRB-binding protein E2F-1

Protein function

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5′-TTTC[CG]CGC-3′ found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters . / BIRC2/c-IAP1 stimulates its transcriptional activity.

Protein sequence and domain

Belongs to the E2F/DP family.

Protein post-translational modifications

Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Phosphorylation at Ser-364 by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis. / Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.

Protein cellular localization

Nucleus

Research area

All research areas>Transcription Regulators>E2F
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize E2F-1 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Rat heart tissue

1: E2F-1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat heart tissue

1: E2F-1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Mouse lung tissue
1: E2F-1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius

1: E2F-1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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