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Rabbit polyclonal EEF2 antibody (A2068)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC,IF
WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:20 – 1:100
Recommended protocols: check protocols
Click or hover above images to see image description for Rabbit polyclonal EEF2 antibody.
Check alternative names for the antibodyExpand
EEF-2 antibody, EF2 antibody
EEF 2 antibody|Eef2 antibody|EF-2 antibody|EF2 antibody|EF2_HUMAN antibody|Elongation factor 2 antibody|Eukaryotic translation elongation factor 2 antibody|Polypeptidyl tRNA translocase antibody|SCA26 antibody|Anti-EEF2 antibody (ab33523)
SCBT cat No: sc-13004|sc-166415|sc-166409|sc-25634|sc-390014|sc-13003|
Rabbit polyclonal EEF2 antibody
|Catalogue No.|| |
Human, Mouse, Rat
Recombinant protein of human EEF2
WB, IHC, IF
|Recommended dilution|| |
WB 1:500 – 1:2000
|Molecular weight|| |
Predicted: 95kDa/Observed: Refer to Figures
EEF2 antibody was tube-contained.
EEF2 antibody was purified using affinity purification.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
EEF-2 antibody, EF2 antibody
|Database links|| |
|Protein names|| |
|Protein function|| |
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
|Protein sequence and domain|| |
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.; Contains 1 tr-type G (guanine nucleotide-binding) domain.
|Protein post-translational modifications|| |
Phosphorylation by EF-2 kinase completely inactivates EF-2; it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase. Phosphorylation by CSK promotes SUMOylation, proteolytic cleavage, and nuclear translocation if the C-terminal fragment.; Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis (By similarity).; ISGylated.; Proteolytically processed at two sites following phosphorylation by CSK.; SUMOylated following phosphorylation by CSK, promotes proteolytic cleavage.
|Protein cellular localization|| |
Cytoplasm . Nucleus . Note: Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product.
Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. It has been shown that phosphorylation of eEF2 at threonine 56 by eEF2 kinase inhibits its activity (1-4). eEF2 kinase is normally dependent on Ca2+ ions and calmodulin (5,6). eEF2 kinase can also be activated by PKA in response to elevated cAMP levels (7-9), which are generally increased in stress- or starvation-related conditions. A variety of treatments known to raise intracellular Ca2+ or cAMP levels have been shown to result in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation. The inactive phosphorylated eEF2 can be converted to its active nonphosphorylated form by a protein phosphatase, most likely a form of protein phosphatase-2A (PP-2A). Insulin, which activates protein synthesis in a wide range of cell types, induces rapid dephosphorylation of eEF2 through mTOR signaling and may involve modulation of the activity of the PP-2A or the eEF2 kinase or both (10).
AntibodyPlus can customize EEF2 antibody according to your requirement, including bulk product size,etc. Please contact firstname.lastname@example.org. AntibodyPlus provides antibody trial sample for your own antibody validation.
|Product type|| |
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