Rabbit polyclonal EEF2K antibody (A5404)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Rabbit polyclonal EEF2K antibody (A5404)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal EEF2K antibody.

Alternative names:

Check alternative names for the antibody

Expand

eEF-2K antibody, HSU93850 antibody
C86191 antibody|Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody|Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody|Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase antibody|Calmodulin dependent protein kinase III antibody|cb365 antibody|EC 2.7.11.20 antibody|eEF 2 kinase antibody|eEF 2K antibody|eEF-2 kinase antibody|eEF-2K antibody|Eef2k antibody|EEF2K protein antibody|EF2K_HUMAN antibody|Elongation factor 2 kinase antibody|Eukaryotic elongation factor 2 kinase antibody|fa04b08 antibody|HSU93850 antibody|kinase eEF2K antibody|MGC45041 antibody|SMEF2K antibody|wu:fa04b08 antibody|zgc:100973 antibody|Anti-EEF2K antibody [EP881Y] (ab45168)

SCBT cat No: sc-393366|sc-390710|sc-21642|sc-21641|sc-514961|sc-398028|sc-398019|sc-21758|sc-377439|sc-68481|sc-517178|

Name

Rabbit polyclonal EEF2K antibody

Catalogue No.

A5404

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human EEF2K

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 82kDa/Observed: Refer to Figures

Formulation

EEF2K antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

EEF2K antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

eEF-2K antibody, HSU93850 antibody

Database links

Human UniProt/Swiss-Prot:O00418

Protein names

eEF-2K, HSU93850

Protein function

Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

Protein sequence and domain

The catalytic domain is located to N-terminal region. The neighbor region contains the calmodulin-binding domain.Belongs to the protein kinase superfamily. Alpha-type protein kinase family.; Contains 1 alpha-type protein kinase domain.

Protein post-translational modifications

Autophosphorylated at multiple residues, Thr-348 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-398 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 at Ser-366 instead decrease EEF2K activity and promote protein synthesis.

Background

This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.

Research area

All research areas>Kinases and Phosphatases>eEF2K
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize EEF2K antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!