Rabbit polyclonal EGLN1 antibody (A1151)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal EGLN1 antibody (A1151)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal EGLN1 antibody.

Alternative names:

Check alternative names for the antibody

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EGLN1 antibody,C1orf12 antibody,DKFZp761F179 antibody,ECYT3 antibody,HIFPH2 antibody,HPH2 antibody,PHD2 antibody,SM-20 antibody,SM20 antibody,ZMYND6 antibody
C1ORF12 antibody|Chromosome 1 Open Reading Frame 12 antibody|DKFZp761F179 antibody|ECYT 3 antibody|ECYT3 antibody|EGL 9 homolog of C. elegans 1 antibody|EGL nine (C.elegans) homolog 1 antibody|Egl nine homolog 1 (C. elegans) antibody|Egl nine homolog 1 antibody|Egl nine like protein 1 antibody|EGLN 1 antibody|Egln1 antibody|EGLN1_HUMAN antibody|HIF PH2 antibody|HIF Prolyl Hydroxylase 2 antibody|HIF-PH2 antibody|HIF-prolyl hydroxylase 2 antibody|HIFP4H 2 antibody|HIFPH2 antibody|HPH 2 antibody|HPH-2 antibody|HPH2 antibody|Hypoxia inducible factor prolyl hydroxylase 2 antibody|Hypoxia-inducible factor prolyl hydroxylase 2 antibody|ORF13 antibody|P4H2 antibody|PHD 2 antibody|PhD2 antibody|PNAS 118 antibody|PNAS 137 antibody|Prolyl Hydroxylase Domain Containing Protein 2 antibody|Prolyl hydroxylase domain-containing protein 2 antibody|Rat Homolog of SM20 antibody|SM 20 antibody|SM-20 antibody|SM20 antibody|Zinc finger MYND domain containing protein 6 antibody|ZMYND6 antibody|Anti-PHD2 / prolyl hydroxylase antibody (ab4561)

SCBT cat No: sc-34920|sc-34921|sc-67030|sc-271835|sc-34923|

Name

Rabbit polyclonal EGLN1 antibody

Catalogue No.

A1151

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human EGLN1

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 46kDa/Observed: Refer to Figures

Formulation

EGLN1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

EGLN1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

EGLN1 antibody,C1orf12 antibody,DKFZp761F179 antibody,ECYT3 antibody,HIFPH2 antibody,HPH2 antibody,PHD2 antibody,SM-20 antibody,SM20 antibody,ZMYND6 antibody

Database links

Human UniProt/Swiss-Prot:Q9GZT9

Protein names

EGLN1,C1orf12,DKFZp761F179,ECYT3,HIFPH2,HPH2,PHD2,SM-20,SM20,ZMYND6

Protein function

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferentially recognized via a LXXLAP motif.

Protein tissue specificity

According to PubMed:11056053, widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. According to PubMed:12351678 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle. According to PubMed:12788921; expressed in adult and fetal heart, brain, liver, lung, skeletal muscle and kidney. Also expressed in placenta. Highest levels in adult heart, brain, lung and liver and fetal brain, heart spleen and skeletal muscle.

Protein sequence and domain

The beta(2)beta(3) ‘finger-like’ loop domain is important for substrate (HIFs’ CODD/NODD) selectivity.Contains 1 Fe2OG dioxygenase domain.; Contains 1 MYND-type zinc finger.

Protein post-translational modifications

S-nitrosylation inhibits the enzyme activity up to 60% under aerobic conditions. Chelation of Fe(2+) has no effect on the S-nitrosylation. It is uncertain whether nitrosylation occurs on Cys-323 or Cys-326.

Protein cellular localization

Cytoplasm . Nucleus . Note: Mainly cytoplasmic. Shuttles between the nucleus and cytoplasm . Nuclear export requires functional XPO1.

Background

PHD1 (Egln2), PHD-2 (Egln1), and PHD3 (Egln3) are members of the Egln family of proline hydroxylases. They function as oxygen sensors that catalyze the hydroxylation of HIF on prolines 564 and 402, initiating the first step of HIF degradation through the VHL/ubiquitin pathway (1,2). PHD1 is highly expressed in a wide array of tissues whereas PHD2 and PHD3 are expressed mainly in heart and skeletal muscle (1,3). The mRNA levels of PHD are upregulated by HIF through the hypoxia-response element under low oxygen conditions (4-7). These three enzymes also exhibit different peptide specificity target proteins, PHD1 and PHD2 can hydroxylate both proline 402 and proline 564, but PHD3 can only hydroxylate proline 564 (2,8). In addition to HIF, PHD enzymes have also has been shown to catalyze the hydroxylation of RNA polymerase subunits and myogenin (3,9).

Note

AntibodyPlus can customize EGLN1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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