Rabbit polyclonal EHMT2 antibody (A1247)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal EHMT2 antibody (A1247)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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EHMT2 antibody,BAT8 antibody,C6orf30 antibody,DKFZp686H08213 antibody,FLJ35547 antibody,G9A antibody,KMT1C antibody,NG36 antibody,NG36/G9a antibody
Ankyrin repeat containing protein antibody|Bat 8 antibody|Bat8 antibody|C6orf30 antibody|DKFZp686H08213 antibody|EHMT 2 antibody|Ehmt2 antibody|EHMT2_HUMAN antibody|Euchromatic histone lysine N methyltransferase 2 antibody|Euchromatic histone-lysine N-methyltransferase 2 antibody|FLJ35547 antibody|G 9a antibody|G9 a antibody|G9a protein antibody|G9A antibody|G9A histone methyltransferase antibody|GAT8 antibody|H3 K9 HMTase 3 antibody|H3-K9-HMTase 3 antibody|Histone H3 K9 methyltransferase 3 antibody|Histone H3 K9 methyltransferase3 antibody|Histone H3-K9 methyltransferase 3 antibody|Histone lysine N methyltransferase antibody|Histone lysine N methyltransferase EHMT2 antibody|Histone lysine N methyltransferase, H3 lysine 9 specific 3 antibody|Histone lysine N methyltransferase, H3 lysine 9 specific3 antibody|Histone-lysine N-methyltransferase EHMT2 antibody|HLA B associated transcript 8 antibody|HLA-B-associated transcript 8 antibody|KMT 1C antibody|KMT1 C antibody|Lysine N methyltransferase 1C antibody|Lysine N-methyltransferase 1C antibody|NG 36 antibody|NG36 antibody|Protein G9a antibody|Anti-EHMT2/G9A antibody [EPR18894] (ab185050)

SCBT cat No: sc-22879|sc-366041|sc-22877|

Name

Rabbit polyclonal EHMT2 antibody

Catalogue No.

A1247

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human EHMT2

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 132kDa/Observed: Refer to Figures

Formulation

EHMT2 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

EHMT2 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

EHMT2 antibody,BAT8 antibody,C6orf30 antibody,DKFZp686H08213 antibody,FLJ35547 antibody,G9A antibody,KMT1C antibody,NG36 antibody,NG36/G9a antibody

Database links

Human UniProt/Swiss-Prot:Q96KQ7

Protein names

EHMT2,BAT8,C6orf30,DKFZp686H08213,FLJ35547,G9A,KMT1C,NG36,NG36/G9a

Protein function

Histone methyltransferase that specifically mono- and dimethylates ‘Lys-9’ of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of ‘Lys-56’ of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates ‘Lys-27’ of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of ‘Lys-373’ of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.

Protein tissue specificity

Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.

Protein sequence and domain

The SET domain mediates interaction with WIZ.; The ANK repeats bind H3K9me1 and H3K9me2.; In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.; Contains 7 ANK repeats.; Contains 1 post-SET domain.; Contains 1 pre-SET domain.; Contains 1 SET domain.

Protein post-translational modifications

Methylated at Lys-185; automethylated.

Protein cellular localization

Nucleus . Chromosome . Note: Associates with euchromatic regions. Does not associate with heterochromatin.

Background

G9a, also known as Euchromatic histone-lysine N-methyltransferase 2 (EHMT2), is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1). Recombinant G9a can mono-, di- and tri-methylate histone H3 on Lys9 and Lys27 in vitro (1,2). However, in vivo G9a forms a complex with GLP, a G9a-related histone methyltransferase, and together these proteins function as the major euchromatic histone H3 Lys9 mono- and di-methyltransferases, creating transcriptionally repressive marks that facilitate gene silencing (3,4). G9a methylates itself on Lys165, a modification that regulates the association of HP1 repressor proteins with the G9a/GLP complex (5). The G9a/GLP complex also contains Wiz, a zinc finger protein that is required for G9a/GLP hetero-dimerization and complex stability (6). Wiz contains two CtBP co-repressor binding sites, which mediate the association of the G9a/GLP with the CtBP co-repressor complex (6). In addition, G9a and GLP are components of other large transcriptional co-repressor complexes, such as those involving E2F6 and CDP/cut (7-9). G9a interacts with DNMT1, and both proteins are required for methylation of DNA and histone H3 (Lys9) at replication foci, providing a functional link between histone H3 Lys9 and CpG methylation during DNA replication (10). G9a activity is critical for meiotic prophase progression, as mutant mice deficient in germ line G9a show a large loss of mature gametes (11). In addition, G9a facilitates increased global levels of di-methyl histone H3 (Lys9) during hypoxic stress and increased G9a expression is associated with hepatocelluar carcinoma (12).

Note

AntibodyPlus can customize EHMT2 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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