Rabbit polyclonal EIF2AK4 antibody (A7155)

$69.00$259.00

Reactivity: Human,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal EIF2AK4 antibody (A7155)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal EIF2AK4 antibody.

Alternative names:

Check alternative names for the antibody

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GCN2 antibody, PVOD2 antibody
E2AK4_HUMAN antibody|Eif2ak4 antibody|Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody|Eukaryotic translation initiation factor 2-alpha kinase 4 antibody|GCN2 antibody|GCN2 eIF2alpha kinase antibody|GCN2 like protein antibody|GCN2-like protein antibody|KIAA1338 antibody|MGCN2 antibody|Phospho anti-GCN2 (T899) antibody [EPR2320Y] (ab75836)

SCBT cat No: sc-46338|sc-374609|sc-66902|

Name

Rabbit polyclonal EIF2AK4 antibody

Catalogue No.

A7155

Reactivity

Human, Rat

Immunogen

Recombinant protein of human EIF2AK4

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 187kDa/Observed: Refer to figures

Formulation

EIF2AK4 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

EIF2AK4 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

GCN2 antibody, PVOD2 antibody

Database links

Human UniProt/Swiss-Prot:Q9P2K8

Protein names

GCN2, PVOD2

Protein function

Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on ‘Ser-52’ in response to low amino acid availability . Plays a role as an activator of the integrated stress response (ISR) required for adapatation to amino acid starvation. Converts phosphorylated eIF-2-alpha/EIF2S1 either to a competitive inhibitor of the translation initiation factor eIF-2B, leading to a global protein synthesis repression, and thus to a reduced overall utilization of amino acids, or to a translational initiation activation of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Binds uncharged tRNAs (By similarity). Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway (UPR) activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation . Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory. Plays a role in neurite outgrowth inhibition. Plays a proapoptotic role in response to glucose deprivation. Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAPK signaling pathways (By similarity). Plays a role in the antiviral response against alphavirus infection; impairs early viral mRNA translation of the incoming genomic virus RNA, thus preventing alphavirus replication (By similarity).; (Microbial infection) Plays a role in modulating the adaptive immune response to yellow fever virus infection; promotes dendritic cells to initiate autophagy and antigene presentation to both CD4(+) and CD8(+) T-cells under amino acid starvation .

Protein tissue specificity

Widely expressed . Expressed in lung, smooth muscle cells and macrophages .

Protein sequence and domain

The histidyl-tRNA synthetase-like region and protein kinase domains are necessary for eIF-2-alpha kinase activity and eIF-2-alpha-mediated translational control. The histidyl-tRNA synthetase-like domain is necessary for binding to uncharged tRNAs. Kinase domain 1 is a degenerate kinase domain.Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.; Contains 2 protein kinase domains.; Contains 1 RWD domain.

Protein post-translational modifications

Autophosphorylated; autophosphorylation on Thr-899 is increased upon amino acid starvation and in UV irradiation cells and inhibited in presence of IMPACT.

Protein cellular localization

Cytoplasm .

Background

This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2.

Research area

All research areas>Kinases and Phosphatases>GCN2
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize EIF2AK4 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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