Rabbit polyclonal EIF4B antibody (A5405)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal EIF4B antibody (A5405)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal EIF4B antibody.

Alternative names:

Check alternative names for the antibody

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EIF-4B antibody, PRO1843 antibody
2310046H11Rik antibody|AL024095 antibody|C85189 antibody|EIF 4B antibody|eIF-4B antibody|EIF4B antibody|Eukaryotic initiation factor 4B antibody|Eukaryotic translation initiation factor 4B antibody|IF4B_HUMAN antibody|PRO1843 antibody|Phospho anti-eIF4B (S422) antibody (ab59300)

SCBT cat No: sc-390912|sc-82586|sc-82587|sc-376062|sc-271480|

Name

Rabbit polyclonal EIF4B antibody

Catalogue No.

A5405

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human EIF4B

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 69kDa/Observed: Refer to Figures

Formulation

EIF4B antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

EIF4B antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

EIF-4B antibody, PRO1843 antibody

Database links

Human UniProt/Swiss-Prot:P23588

Protein names

EIF-4B, PRO1843

Protein function

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5′-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Protein sequence and domain

Contains 1 RRM (RNA recognition motif) domain.

Protein post-translational modifications

Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.

Background

Eukaryotic initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly-(A) binding protein, increasing its poly-(A) binding activity (1). Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation (2-5). Multiple kinases, including p70 S6 kinase, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002 (6). Recently, Ser406 was identified as a novel phosphorylation site regulated by mitogens (7), and the phosphorylation of this site is dependent on MEK and mTOR activity (7). This phosphorylation is shown to be essential for the translational activity of eIF4B (7).

Research area

All research areas>Signaling Intermediates>eIF
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus can customize EIF4B antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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