Rabbit polyclonal EIF4E antibody (A2162)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,ICC,IP
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal EIF4E antibody (A2162)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,ICC,IP

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:100 ICC 1:50 – 1:200 IP 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal EIF4E antibody.

Alternative names:

Check alternative names for the antibody

Expand

CBP antibody, EIF4E1 antibody, EIF4EL1 antibody, EIF4F antibody, MGC111573 antibody
AUTS19 antibody|CBP antibody|eIF 4E antibody|eIF 4F 25 kDa subunit antibody|EIF 4F antibody|eIF-4E antibody|eIF-4F 25 kDa subunit antibody|eIF4E antibody|EIF4E1 antibody|EIF4EL1 antibody|EIF4F antibody|Eukaryotic translation initiation factor 4 E antibody|Eukaryotic translation initiation factor 4E antibody|Eukaryotic translation initiation factor 4E like 1 antibody|IF4E_HUMAN antibody|Messanger RNA Cap Binding Protein eIF 4E antibody|MGC111573 antibody|mRNA cap binding protein antibody|mRNA cap-binding protein antibody|Phospho anti-eIF4E (S209) antibody [EP2151Y] (ab76256)

SCBT cat No: sc-271480|sc-6968|sc-514875|sc-13963|sc-9976|sc-100731|

Name

Rabbit polyclonal EIF4E antibody

Catalogue No.

A2162

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human EIF4E

Host

Rabbit

Applications

WB, IHC, ICC, IP

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:100
ICC 1:50 – 1:200
IP 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 29kDa/Observed: Refer to Figures

Formulation

EIF4E antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

EIF4E antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CBP antibody, EIF4E1 antibody, EIF4EL1 antibody, EIF4F antibody, MGC111573 antibody

Database links

Human UniProt/Swiss-Prot:P06730

Protein names

CBP, EIF4E1, EIF4EL1, EIF4F, MGC111573

Protein function

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

Protein sequence and domain

Belongs to the eukaryotic initiation factor 4E family.

Protein post-translational modifications

Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.

Protein cellular localization

Cytoplasm, P-body . Cytoplasm .

Background

Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148, and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin.

Research area

All research areas>Signaling Intermediates>eIF
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus can customize EIF4E antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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