Rabbit polyclonal ENOX2 antibody (A7063)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal ENOX2 antibody (A7063)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal ENOX2 antibody.

Alternative names:

Check alternative names for the antibody

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APK1 antibody, tNOX antibody, COVA1 antibody
APK1 antibody|APK1 antigen antibody|COVA1 antibody|Cytosolic ovarian carcinoma antigen 1 antibody|Ecto NOX disulfide thiol exchanger 2 antibody|ENOX 2 antibody|ENOX2 antibody|ENOX2_HUMAN antibody|OTTHUMP00000024021 antibody|OTTHUMP00000024022 antibody|Protein disulfide-thiol oxidoreductase antibody|tNOX antibody|Tumor associated hydroquinone oxidase antibody|Tumor-associated hydroquinone oxidase antibody|Anti-ENOX2 antibody (ab210751)

SCBT cat No:

Name

Rabbit polyclonal ENOX2 antibody

Catalogue No.

A7063

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human ENOX2

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 70kDa/Observed: Refer to figures

Formulation

ENOX2 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

ENOX2 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

APK1 antibody, tNOX antibody, COVA1 antibody

Database links

Human UniProt/Swiss-Prot:Q16206

Protein names

APK1, tNOX, COVA1

Protein function

May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock.

Protein tissue specificity

Found in the sera of cancer patients with a wide variety of cancers including breast, prostate, lung and ovarian cancers, leukemias, and lymphomas. Not found in the serum of healthy volunteers or patients with disorders other than cancer. Probably shed into serum by cancer cells. Found on the cell borders of renal, kidney and ovarian carcinomas but not on the borders of surrounding non-cancerous stromal cells.

Protein sequence and domain

Belongs to the ENOX family.; Contains 1 RRM (RNA recognition motif) domain.

Protein post-translational modifications

Glycosylated.

Protein cellular localization

Cell membrane. Secreted, extracellular space. Note: Extracellular and plasma membrane-associated.

Background

This gene is a tumor-specific member of the ECTO-NOX family of genes that encode cell surface NADH oxidases. The encoded protein has two enzymatic activities: catalysis of hydroquinone or NADH oxidation, and protein disulfide interchange. The protein also displays prion-like properties. Alternative splicing results in multiple transcript variants.

Note

AntibodyPlus can customize ENOX2 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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Antibody customer reviews & validation data - 110

Validation data ID: 110

Submission date: 2017-02-10

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Result figure:
Result figure

Figure description:

Immunoblotting detection of ENOX2 in total lysates of hTERT-RPE1 cells

Product name

Rabbit polyclonal ENOX2 antibody

Product catalog No.

A7063

Product type

Primary antibody

Supplier

St John's Laboratory Ltd

Application type

Western Blot

Antibody specificity rating

Excellent

Antibody overall rating

Excellent

Your comments

We tested the ENOX2 antibody on western blots with total lysates of human retinal epithelial cells (hTert-RPE1, ATCC), both wt and edited by CRISPR/Cas9 to delete ENOX2.
Conditions: BioRad minigels 4-20%, 15 wells, with 5-20 micograms/well. Blotting: BioRad Transblot Turbo, PVDF membrane. Block: 5% nonfat dried milk or BioRad blocker. First antibody 1:1000, overnight at 4C, anti-rabbit/HPX 1:5000, 20 min at room temp. Developed with ChemiBlot XRS BioRad imager. Exposure 10-120 seconds. This test was repeated 3 times.
In the wt lysates, the antibody detected 2 bands, about 66 kDa and about 55 kDa. No signal was detected in the ENOX2-deleted lysates.
Virtually the same pattern was detected with another commercial ENOX2 antibody (LSBio) in the same samples.
In immmunofluorescence in the wt hTERT-RPE1 cells (1:50 or 1:100), the antibody gave a weak nuclear and cytoplasmic signal. This test was done only once.

Detailed protocol

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